The Regulatory Roles of ncRNA Rli60 in Adaptability of Listeria monocytogenes to Environmental Stress and Biofilm Formation

文献类型： 外文期刊

第一作者： Peng, Ye-long

作者： Peng, Ye-long;Meng, Qing-ling;Qiao, Jun;Xie, Kun;Chen, Cheng;Liu, Tian-li;Hu, Zheng-xiang;Ma, Yu;Chen, Chuang-fu;Cai, Xue-peng

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期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

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收录情况： SCI

摘要： Listeria monocytogenes is a facultative anaerobic Gram-positive bacterium. It is well adapted to external environments and able to infect both humans and animals. To understand the impacts of ncRNA Rli60 on the adaptability of L. monocytogenes to environmental stresses and biofilm formation, a rli60 deletion strain of L. monocytogenes (LM-Delta rli60) was constructed using splicing by overlap extension PCR (SOE-PCR) and homologous recombination and then compared it with wild-type strain L. monocytogenes EGD-e in the aspects of adaptability to environmental stresses by measuring their growth under stresses of different temperatures, and acidic, alkaline, hypertonic and alcoholic conditions, and capability of biofilm formation by using crystal violet staining, as well as the transcriptional levels of genes (gltB and gltC) related to the biofilm formation by real-time quantitative PCR (qRT-PCR). The results showed that (1) the growth of LM-Delta rli60 strain was significantly slower under environmental stresses of low temperature (30 A degrees C), high temperature (42 A degrees C), as well as alkaline and alcoholic conditions, (2) the amount of biofilm formed by LM-Delta rli60 was attenuated, and (3) the transcriptional levels of gltB and gltC genes at 24 h and 48 h in LM-Delta rli60 revealed a significant reduction. Overall, the results confirmed that ncRNA Rli60 plays important roles in regulating the adaptability of L. monocytogenes to environmental stresses and biofilm formation possibly through impacting the expression of gltB and gltC genes.

分类号： Q93

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