Genetic improvement of the nematicidal fungus Lecanicillium attenuatum against Heterodera glycines by expression of the Beauveria bassiana Cdep1 protease gene

文献类型: 外文期刊

第一作者: Zhang, Yan-Jun

作者: Zhang, Yan-Jun;Zhang, Xiao-Lin;Peng, De-Liang;Yu, Wen-Bin;Li, Qian

作者机构:

关键词: Nematophagous fungi;Genetic engineering;Protease;Soybean cyst nematode

期刊名称:JOURNAL OF INVERTEBRATE PATHOLOGY ( 影响因子:2.841; 五年影响因子:3.368 )

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收录情况: SCI

摘要: Lecanicillium attenuatum is an important nematophagous fungus with potential as a biopesticide against plant-parasitic nematodes. The Pr1A-like cuticle-degrading protease (Cdep1) gene originating from the entomopathogenic fungus Beauveria bassiana was transformed into the nematophagous fungus L. attenuatum using a polyethylene-glycol mediated protoplast-based transformation system. Protease activity was increased 0.64- to 1.63-fold 2-10 d after growth in the transformed L. attenuatum. Inhibition of egg-hatching and J2 motility of soybean cyst nematodes (Heterodera glycines) by cell-free fungal culture filtrates were enhanced by 17-76% 2-14 d and 43-152% 1-13 d after incubation, respectively. (C) 2016 Elsevier Inc. All rights reserved.

分类号: Q959.1

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