Preparative isolation of cordycepin, N-6-(2-hydroxyethyl)-adenosine and adenosine from Cordyceps militaris by macroporous resin and purification by recycling high-speed counter-current chromatography

文献类型： 外文期刊

第一作者： Zhang, Zhong

作者： Zhang, Zhong;Tudi, Tuernisan;Liu, Yanfang;Zhou, Shuai;Feng, Na;Yang, Yan;Tang, Chuanhong;Tang, Qingjiu;Zhang, Jingsong;Tudi, Tuernisan;Tang, Qingjiu

作者机构：

关键词： Cordyceps militaris;Macroporous resin;High-speed counter-current chromatography;Cordycepin;N-6-(2-hydroxyethyl)-adenosine;Adenosine

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： In this study, cordycepin, N-6-(2-hydroxyethyl)-adenosine (HEA) and adenosine from the fruiting bodies of Cordyceps militaris were separated by using macroporous resin NKA-II adsorption. The parameters of static adsorption were tested and the optimized conditions were as follow: the total adsorption time was 12 h, 50% ethanol was used for desorption and the desorption time was 9 h. The crude sample that was prepared by macroporous resin NKA-II contained 3.4% cordycepin, 3.7% HEA and 4.9% adenosine. Then the crude sample was further purified by recycling high-speed counter-current chromatography (HSCCC) with ethyl acetate, n-butanol, 1.5% aqueous ammonium hydroxide (1:4:5, v/v/v) as the optimized two-phase solvent system. Three nucleosides including 15.6 mg of cordycepin, 16.9 mg of HEA and 23.2 mg of adenosine were obtained from 500 mg of crude sample in one-step separation. The purities of three compounds were 98.5, 98.3 and 98.0%, respectively, as determined by high performance liquid chromatography. (C) 2016 Published by Elsevier B.V.

分类号： Q503

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