Proteomic Analysis of Mouse Oocytes Identifies PRMT7 as a Reprogramming Factor that Replaces SOX2 in the Induction of Pluripotent Stem Cells

文献类型： 外文期刊

第一作者： Wang, Bingyuan

作者： Wang, Bingyuan;Pfeiffer, Martin J.;Boiani, Michele;Drexler, Hannes C. A.;Fuellen, Georg

作者机构：

关键词： development;DNMT3A;induced pluripotent stem cells;oocyte proteome;PRMT7;reprogramming;reprogrammome;SILAC;STELLA

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The reprogramming process that leads to induced pluripotent stem cells (iPSCs) may benefit from adding oocyte factors to Yamanaka's reprogramming cocktail (OCT4, SOX2, KLF4, with or without MYC; OSK(M)). We previously searched for such facilitators of reprogramming (the reprogrammome) by applying label-free LC-MS/MS analysis to mouse oocytes, producing a catalog of 28 candidates that are (i) able to robustly access the cell nucleus and (ii) shared between mature mouse oocytes and pluripotent embryonic stem cells. In the present study, we hypothesized that our 28 reprogrammome candidates would also be (iii) abundant in mature oocytes, (iv) depleted after the oocyte-to-embryo transition, and (v) able to potentiate or replace the OSKM factors. Using LC-MS/MS and isotopic labeling methods, we found that the abundance profiles of the 28 proteins were below those of known oocyte-specific and housekeeping proteins. Of the 28 proteins, only arginine methyltransferase 7 (PRMT7) changed substantially during mouse embryogenesis and promoted the conversion of mouse fibroblasts into iPSCs. Specifically, PRMT7 replaced SOX2 in a factor-substitution assay, yielding iPSCs. These findings exemplify how proteomics can be used to prioritize the functional analysis of reprogrammome candidates. The LC-MS/MS data are available via ProteomeXchange with identifier PXD003093.

分类号： Q7`Q51

  • 相关文献

[1]Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer. Yao, Ya-Xin,Li, He-Ping,Yao, Ya-Xin,Guan, Wei-Jun,Li, Xiang-Chen,Su, Xiao-Hua,Ma, Yue-Hui. 2012

[2]Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos. Huan, Yan Jun,Wang, Hong Mei,Bin He, Hong,Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Li, Jing Yu,Kong, Qing Ran,Liu, Zhong Hua,Zhu, Jiang,Wu, Zhan Feng,Zhang, Ji Guang.

[3]Differential gene expression in mouse spermatogonial stem cells and embryonic stem cells. Bai, Yinshan,Liu, Shanshan,Ma, Ningfang,Bai, Yinshan,Feng, Meiying,Wei, Hengxi,Li, Li,Zhang, Xianwei,Shen, Chao,Zhang, Shouquan.

[4]Melatonin improves reprogramming efficiency and proliferation of bovine-induced pluripotent stem cells. Bai, Chunyu,Li, Xiangchen,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing,Guan, Weijun,Ma, Yuehui,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing. 2016

[5]Recent advances in the development of new transgenic animal technology. Miao, Xiangyang,Miao, Xiangyang.

[6]Construction of recombinant proteins for reprogramming of endangered Luxi cattle fibroblast cells. Hu, P. F.,Guan, W. J.,Li, X. C.,Ma, Y. H..

[7]Murine pluripotent stem cells that escape differentiation inside teratomas maintain pluripotency. Pei, Yangli,Pei, Yangli,Yue, Liang,Zhang, Wei,Xiang, Jinzhu,Han, Jianyong,Ma, Zhu. 2018

[8]Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors. Du, Xuguang,Feng, Tao,Yu, Dawei,Zou, Huiying,Ma, Shuangyu,Hu, Xiaoxiang,Li, Ning,Wu, Sen,Wu, Yuanyuan,Feng, Chong,Pan, Dengke,Huang, Yongye,Ouyang, Hongsheng.

[9]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[10]Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation. Kang Liu,Meiling Han,Chaojun Zhang,Liangyu Yao,Jing Sun,Tianzhen Zhang.

[11]An NAM Domain Gene, GhNAC79, Improves Resistance to Drought Stress in Upland Cotton. Guo, Yaning,Yu, Shuxun,Jia, Xiaoyun,Dou, Lingling,Yu, Shuxun,Guo, Yaning,Pang, Chaoyou,Ma, Qifeng,Zhao, Fengli,Gu, Lijiao,Wei, Hengling,Wang, Hantao,Fan, Shuli,Su, Junji. 2017

[12]Genome-wide analysis of the WRKY gene family in cotton. Dou, Lingling,Zhang, Xiaohong,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Yu, Shuxun.

[13]Body color development and genetic analysis of hybrid transparent crucian carp (Carassius auratus). Xu, W.,Tong, G. X.,Geng, L. W.,Jiang, H. F.. 2015

[14]The Current State and Future Strategy for Utilizing Biomass Energy in Beijing. Zhou, Zhongren,Wang, Ailing,Zhou, Liandi,Chen, Qun. 2012

[15]Optimization and evaluation of microencapsulated artificial diet for mass rearing the predatory ladybird Propylea japonica (Coleoptera: Coccinellidae). Tan, Xiao-Ling,Wang, Su,Zhang, Fan,Tan, Xiao-Ling,Zhao, Jing. 2015

[16]Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs. Yang, Yalan,Sun, Wei,Wang, Ruiqi,Zhou, Rong,Tang, Zhonglin,Li, Kui,Yang, Yalan,Tang, Zhonglin,Li, Kui,Sun, Wei,Lei, Chuzhao. 2015

[17]Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum. Dao, Jinwei,Zhu, Lihui,Luo, Rong,Hu, Chao,Wang, Yuqing,Li, Hao,Lu, Ke,Liu, Jinming,Lin, Jiaojiao,Cheng, Guofeng,Dao, Jinwei,Cheng, Guofeng. 2014

[18]RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Zhou, Li-Tao,Jia, Shuang,Wan, Pin-Jun,Kong, Ye,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[19]Expression Profiling of miRNA-122 and miRNA-221 in Porcine Various Tissues and Developing Testes. Lian, Chuanjiang,Niu, Shuling,Yang, Runjun,Sun, Boxing,Shen, Binglei,Liu, Boyang,Zhang, Liying,Zhao, Zhihui,Qin, Lihong,Bai, Wenlin. 2011

[20]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

作者其他论文 更多>>

微信小程序