Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein
文献类型: 外文期刊
第一作者: Song, Puwen
作者: Song, Puwen;Wu, Bingyue;Cui, Xiaoyan;Chen, Xin;Zhi, Haijian
作者机构:
关键词: Interaction;Soybean mosaic virus;P3N-PIPO;Golgi SNARE 12;Yeast two hybrid;Bimolecular fluorescence complementation
期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )
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收录情况: SCI
摘要: Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus. (C) 2016 Elsevier Inc. All rights reserved.
分类号: Q5
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