A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products

文献类型： 外文期刊

第一作者： Majdinasab, Marjan

作者： Majdinasab, Marjan;Li, Peiwu;Zhang, Qi;Li, Xin;Tang, Xiaoqian;Li, Jing;Majdinasab, Marjan;Sheikh-Zeinoddin, Mahmoud;Soleimanian-Zad, Sabihe;Li, Peiwu;Zhang, Qi;Li, Xin;Tang, Xiaoqian;Li, Jing;Li, Peiwu;Li, Peiwu

作者机构：

关键词： Time-resolved fluorescent immunochromatographic assay (TRFICA);Ochratoxin A;Agro-products;Carboxylate-modified Eu MO-polystyrene nanoparticles

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Immunochromatographic assays (ICAs) are considered as a suitable diagnostic tool for the detection of mycotoxins. Mycotoxins and especially, ochratoxin A are analytes with more demanding sensitivity requirements. To enhance the sensitivity of current immunochromatographic assays for ochratoxin A (OTA), a novel sensitive ICA was developed in this study. In the assay, microspheres enclosing fluorescent europium (III) [Eu(III)] nanoparticles (EuNPs) were used as a label for OTA monoclonal antibody (OTA-mAb) conjugation. Accordingly, assay was called time-resolved fluorescent immunochromatographic assay (TRFICA). The test strip was composed of three parts: a sample pad, nitrocellulose membrane and an absorbent pad. As for detection, a proper concentration of conjugated microspheres was pipetted into the microtube and sample extract was added to it. Then the strip was inserted into the tube and the fluid flow along the strip. The TRFICA results were obtained in 8 min and read by a portable TRFICA strip reader. The established method allows quantitative determination of OTA with limit of detection as low as 1.0 mu g kg(-1) in the samples. For validation, spiked samples including wheat, maize, soybean and rice were respectively assayed by TRFICA and a standard high performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD), and good agreement of results was obtained between two methods. (C) 2014 Elsevier Ltd. All rights reserved.

分类号： TS2

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