Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J

文献类型: 外文期刊

第一作者: Dai, Zhenkai

作者: Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Zhang, Huanmin;Lin, Wencheng;Chen, Weiguo;Xie, Qingmei

作者机构:

关键词: DNA vaccine;Avian leucosis virus;Autophagy;Rapamycin

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old-chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85 + rapamycin and pVAX1-gp85-LC3 + rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-gamma expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+ CD8+ T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J. (C) 2016 Elsevier Ltd. All rights reserved.

分类号: R392

  • 相关文献

[1]Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Guo, X.,Dong, Y.,Yin, S.,Zhao, C.,Huo, Y.,Hu, H.,Guo, X.,Fan, L.. 2013

[2]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

[3]Rice Stripe Virus Interferes with S-acylation of Remorin and Induces Its Autophagic Degradation to Facilitate Virus Infection. Xu, Yi,Li, Chenyang,Wu, Jianxiang,Zhou, Xueping,Li, Chenyang,Zhou, Xueping,Li, Yi,Xu, Yi. 2018

[4]Autophagy Plays an Important Role in Anti-inflammatory Mechanisms Stimulated by Alpha7 Nicotinic Acetylcholine Receptor. Shao, Bo-Zong,Ke, Ping,Xu, Zhe-Qi,Cheng, Ming-He,Han, Bin-Ze,Su, Ding-Feng,Liu, Chong,Wei, Wei,Chen, Xiong-Wen. 2017

[5]Regulation of ATG6/Beclin-1 homologs by abiotic stresses and hormones in rice (Oryza sativa L.). Rana, R. M.,Dong, S.,Huang, J.,Zhang, H. S.,Rana, R. M.,Ali, Z.,Ali, Z.. 2012

[6]3 '-epi-12 beta-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Mei, Wen-Li,Dai, Hao-Fu,Liu, Quan,Liu, Quan,Huang, Canhua,Li, Yue-Nan. 2018

[7]Activating Cannabinoid Receptor 2 Alleviates Pathogenesis of Experimental Autoimmune Encephalomyelitis Via Activation of Autophagy and Inhibiting NLRP3 Inflammasome. Shao, Bo-Zong,Ke, Ping,Xu, Zhe-Qi,Zhou, Jv-Xiang,Liu, Chong,Wei, Wei. 2014

[8]Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides. Yu, Xinmao,Zhou, Yongzhi,Cao, Jie,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[9]Autophagy regulates spermatid differentiation via degradation of PDLIM1. Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Liu, Weixiao,Song, Zhenhua,Xu, Zhiliang,Li, Wei,Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Song, Zhenhua,Xu, Zhiliang,Jia, Pengfei,Yang, Lin,Jia, Pengfei,Yang, Lin,Wang, Yanfang.

[10]Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy. Liu, Jia,Zheng, Lanhong,Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Ma, Leina,Wang, Bin,Zhao, Youguang,Lin, Xiukun.

[11]Infectious Bursal Disease Virus Subverts Autophagic Vacuoles To Promote Viral Maturation and Release. Wang, Yongqiang,Duan, Yulu,Han, Chunyan,Yao, Shuai,Qi, Xiaole,Gao, Yulong,Zhang, Lizhou,Gao, Li,Gao, Honglei,Shen, Nan,Wang, Jingfei,Wang, Xiaomei,Maier, Helena J.,Britton, Paul,Chen, Lei.

[12]An efficient virus-induced gene silencing vector for maize functional genomics research. Wang, Rong,Yang, Xinxin,Wang, Nian,Liu, Xuedong,Fan, Zaifeng,Zhou, Tao,Wang, Rong,Yang, Xinxin,Wang, Nian,Liu, Xuedong,Fan, Zaifeng,Zhou, Tao,Nelson, Richard S.,Li, Weimin.

[13]Thapsigargin induces apoptosis when autophagy is inhibited in HepG2 cells and both processes are regulated by ROS-dependent pathway. Wang, Congcong,Tang, Shusheng,Zhao, Dongxu,Zhang, Chaoming,Zhang, Shen,Deng, Sijun,Zhou, Yan,Xiao, Xilong,Wang, Congcong,Li, Tao.

[14]Oleanolic acid induces protective autophagy in cancer cells through the JNK and mTOR pathways. Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Zheng, Lanhong,Liu, Jia,Liu, Ge,Lin, Xiukun,Zhong, Jiateng.

[15]Comparative study of two immunity-related GTPase genes in Chinese soft-shell turtle reveals their molecular characteristics and functional activity in immune defense. Shi, Yan,Hu, Sufei,Ding, Tie,Zhao, Zhe,Shi, Yan,Shen, Sixian,Hong, Xiaoyou,Chen, Chen,Zhu, Xinping. 2018

[16]Responses of Plant Proteins to Heavy Metal Stress-A Review. Hasan, Md. Kamrul,Kanwar, Mukesh K.,Ahammed, Golam J.,Hasan, Md. Kamrul,Cheng, Yuan,Chu, Xian-Yao,Qi, Zhen-Yu. 2017

[17]Autophagy in Negative-Strand RNA Virus Infection. Wang, Yupeng,Jiang, Ke,Meng, Songshu,Zhang, Quan,Ding, Chan. 2018

[18]PI3K-Akt-mTOR axis sustains rotavirus infection via the 4E-BP1 mediated autophagy pathway and represents an antiviral target. Yin, Yuebang,Dang, Wen,Zhou, Xinying,Xu, Lei,Wang, Wenshi,Cao, Wanlu,Chen, Sunrui,Peppelenbosch, Maikel P.,Pan, Qiuwei,Su, Junhong,Cai, Xuepeng,Xiao, Shaobo. 2018

[19]Autophagy Activated by Bluetongue Virus Infection Plays a Positive Role in Its Replication. Lv, Shuang,Xu, Qingyuan,Sun, Encheng,Yang, Tao,Li, Junping,Feng, Yufei,Zhang, Qin,Wang, Haixiu,Zhang, Jikai,Wu, Donglai. 2015

[20]Impaired Cellular Energy Metabolism Contributes to Duck-Enteritis-Virus-Induced Autophagy via the AMPK-TSC2-MTOR Signaling Pathway. Yin, Haichang,Zhao, Lili,Li, Siqi,Xu, Lijing,Wang, Yiping,Chen, Hongyan. 2017

作者其他论文 更多>>