Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J
文献类型: 外文期刊
第一作者: Dai, Zhenkai
作者: Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Dai, Zhenkai;Huang, Jianfei;Lei, Xiaoya;Yan, Yiming;Lu, Piaopiao;Lin, Wencheng;Chen, Weiguo;Ma, Jingyun;Xie, Qingmei;Zhang, Huanmin;Lin, Wencheng;Chen, Weiguo;Xie, Qingmei
作者机构:
关键词: DNA vaccine;Avian leucosis virus;Autophagy;Rapamycin
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old-chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85 + rapamycin and pVAX1-gp85-LC3 + rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-gamma expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+ CD8+ T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J. (C) 2016 Elsevier Ltd. All rights reserved.
分类号: R392
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