Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation

文献类型: 外文期刊

第一作者: Zhou, Chun-Xue

作者: Zhou, Chun-Xue;Suo, Xun;Zhou, Chun-Xue;Suo, Xun;Zhou, Chun-Xue;Zhu, Xing-Quan;He, Shuai;Zhou, Dong-Hui;Elsheikha, Hany M.;He, Shuai;Li, Qian

作者机构:

关键词: Toxoplasma gondii;Oocyst;Sporulation;iTRAQ;Proteomics

期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )

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收录情况: SCI

摘要: Toxoplasma gondii is a medically and economically important protozoan parasite. However, the molecular mechanisms of its sporulation remain largely unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the proteomic expression profile of T. gondii oocysts during sporulation. Of the 2095 non-redundant proteins identified, 587 were identified as differentially expressed proteins (DEPs). Based on Gene Ontology enrichment and KEGG pathway analyses the majority of these DEPs were found related to the metabolism of amino acids, carbon and energy. Protein interaction network analysis generated by STRING identified ATP-citrate lyase (ACL), GMP synthase, IMP dehydrogenase (IMPDH), poly (ADP-ribose) glycohydrolase (PARG), and bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) as the top five hubs. We also identified 25 parasite virulence factors that were expressed at relatively high levels in sporulated oocysts compared to non-sporulated oocysts, which might contribute to the infectivity of mature oocysts. Considering the importance of oocysts in the dissemination of toxoplasmosis these findings may help in the search of protein targets with a key role in infectiousness and ecological success of oocysts, creating new opportunities for the development of better means for disease prevention.

分类号: Q51

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