The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes

文献类型： 外文期刊

第一作者： Xu, Haifeng

作者： Xu, Haifeng;Wang, Nan;Liu, Jingxuan;Qu, Changzhi;Wang, Yicheng;Jiang, Shenghui;Wang, Deyun;Zhang, Zongying;Chen, Xuesen;Lu, Ninglin

作者机构：

关键词： Malus sieversii f. neidzwetzkyana;First-filial generation;Anthocyanin;MdMYB16;MdbHLH33;EAR suppression sequence;Homodimers

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Anthocyanins are strong antioxidants that play a key role in the prevention of cardiovascular disease, cancer, and diabetes. The germplasm of Malus sieversii f. neidzwetzkyana is important for the study of anthocyanin metabolism. To date, only limited studies have examined the negative regulatory mechanisms underlying anthocyanin synthesis in apple. Here, we analyzed the relationship between anthocyanin levels and MdMYB16 expression in mature Red Crisp 1-5 apple (M. domestica) fruit, generated an evolutionary tree, and identified an EAR suppression sequence and a bHLH binding motif of the MdMYB16 protein using protein sequence analyses. Overexpression of MdMYB16 or MdMYB16 without bHLH binding sequence (LBSMdMYB16) in red-fleshed callus inhibited MdUFGT and MdANS expression and anthocyanin synthesis. However, overexpression of MdMYB16 without the EAR sequence (LESMdMYB16) in red-fleshed callus had no inhibitory effect on anthocyanin. The yeast one-hybrid assay showed that MdMYB16 and LESMdMYB16 interacted the promoters of MdANS and MdUFGT, respectively. Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays showed that MdMYB16 formed homodimers and interacted with MdbHLH33, however, the LBSMdMYB16 could not interact with MdbHLH33. We overexpressed MdbHLH33 in callus overexpressing MdMYB16 and found that it weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis. Together, these results suggested that MdMYB16 and MdbHLH33 may be important part of the regulatory network controlling the anthocyanin biosynthetic pathway.

分类号： Q946

  • 相关文献

[1]The Synthesis and Accumulation of Resveratrol Are Associated with Veraison and Abscisic Acid Concentration in Beihong (Vitis vinifera x Vitis amurensis) Berry Skin. Wang, Junfang,Wang, Shuqin,Liu, Guotian,Duan, Wei,Li, Shaohua,Wang, Lijun,Wang, Junfang,Wang, Shuqin,Liu, Guotian,Duan, Wei,Li, Shaohua,Wang, Lijun,Wang, Junfang,Wang, Shuqin,Liu, Guotian,Edwards, Everard J.. 2016

[2]Characterization and evaluation of major anthocyanins in pomegranate (Punica granatum L.) peel of different cultivars and their development phases. Zhao, Xueqing,Fang, Yanming,Zhao, Xueqing,Yuan, Zhaohe,Yin, Yanlei,Feng, Lijuan. 2013

[3]Comparative Study of Phenolic Compounds and Antioxidant Activity in Different Species of Cherries. Liu, Yun,Zhong, Fei,Tian, Rongrong,Li, Tianhong,Liu, Xinyan,Zhang, Kaichun,Zhang, Xiaoming. 2011

[4]Response of phenolic compounds in 'Golden Delicious' and 'Red Delicious' apples peel to fruit bagging and subsequent sunlight re-exposure. Sun, Shan,Xin, Li,Gao, Huajun,Wang, Jiangyong,Li, Pengmin. 2014

[5]A key structural gene, AaLDOX, is involved in anthocyanin biosynthesis in all red-fleshed kiwifruit (Actinidia arguta) based on transcriptome analysis. Li, Yukuo,Fang, Jinbao,Qi, Xiujuan,Lin, Miaomiao,Zhong, Yunpeng,Sun, Leiming. 2018

[6]Physical changes and physiological characteristics of red and green peel during nectarine (cv. Hu018) maturation. Su, Mingshen,Chen, Kunsong,Zhang, Bo,Xu, Changjie,Sun, Chongde,Zhang, Jiukai,Li, Xian,Xi, Wanpeng,Su, Mingshen,Ye, Zhengwen,Wu, Aizhong,Guo, Juan. 2012

[7]Comparative analysis of pigments in red and yellow banana fruit. Cheng, Sihua,Yang, Ziyin,Huang, Bingzhi,Du, Bing,Zeng, Wei. 2018

[8]Molecular cloning and gene expression differences of the anthocyanin biosynthesis-related genes in the red/green skin color mutant of pear (Pyrus communis L.). Yang, Ya-nan,Zhao, Guang,Zhang, Shao-ling,Gu, Chao,Wu, Jun,Yue, Wen-quan. 2013

[9]Quantification and recovery of anthocyanins from litchi pericarps. Liu Xueming,Zhang Mingwei,Chen Zhiyi,Shi Ying,Zou Yuxiao. 2013

[10]Responses of hypocotyl elongation to light and sowing depth in peanut seedlings. Xu, Hui-lian,Qin, Feifei,Takano, Tetsuo,Qin, Feifei,Lu, Dianqiu. 2012

[11]Thermal characterization of the anthocyanins from black soybean (Glycine max L.) exposed to thermogravimetry. Zhao, Xiaoyan. 2014

[12]Mining for Candidate Genes in an Introgression Line by Using RNA Sequencing: The Anthocyanin Overaccumulation Phenotype in Brassica. Xie, Lulu,Li, Fei,Zhang, Shifan,Zhang, Hui,Qian, Wei,Li, Peirong,Zhang, Shujiang,Sun, Rifei. 2016

[13]Genistein: A Novel Anthocyanin Synthesis Promoter that Directly Regulates Biosynthetic Genes in Red Cabbage in a Light-Dependent Way. Zhang, Na,Qi, Yan,Wang, Xiaoyun,Li, Hongfei,Guo, Yang-Dong,Qi, Yan,Shi, Yantong,Zhang, Hai-Jun. 2016

[14]Changes in Content and Component of Purple Corn (Zea Mays L.) Anthocyanin during the Extraction and Preparation. Wang, D.. 2015

[15]Effects of fruit bagging on coloring and related physiology, and qualities of red Chinese sand pears during fruit maturation. Huang, Chunhui,Yu, Bo,Teng, Yuanwen,Su, Jun,Shu, Qun,Cheng, Zaiquan,Zeng, Liqiong. 2009

[16]Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in 'Yunhongli No. 1' (Pyrus pyrifolia Nakai) pears. Zhang, Dong,Yu, Bo,Qian, Minjie,Teng, Yuanwen,Bai, Jinhe,Shu, Qun,Su, Jun. 2012

[17]Phenolic compounds participating in mulberry juice sediment formation during storage. Zou, Bo,Xu, Yu-juan,Wu, Ji-jun,Yu, Yuan-shan,Xiao, Geng-sheng. 2017

[18]Calf Thymus DNA-Binding Ability Study of Anthocyanins from Purple Sweet Potatoes (Ipomoea batatas L.). Wang, Dan,Wang, Xirui,Zhang, Chao,Ma, Yue,Zhao, Xiaoyan,Wang, Xirui. 2011

[19]Changes in seed growth, levels and distribution of flavonoids during tartary buckwheat seed development. Song, Chao,Xiang, Da-Bing,Yan, Lin,Song, Yue,Zhao, Gang,Wang, Yue-Hua,Zhang, Bao-Lin. 2016

[20]Different light-response patterns of coloration and related gene expression in red pears (Pyrus L.). Zhu, Yang-fan,Yao, Gai-fang,Liu, Hai-nan,Gu, Chao,Qin, Meng-fan,Bai, Bing,Cai, Shao-shuai,Wang, Guo-ming,Wang, Run-ze,Wu, Jun,Su, Jun,Shu, Qun. 2018

作者其他论文 更多>>

微信小程序