TALEN construction for porcine I kappa B alpha gene and the detection of knockout activity

文献类型： 外文期刊

第一作者： Xu, Kui

作者： Xu, Kui;Ruan, Jinxue;Li, Hegang;Wu, Tianwen;Liang, Bo;Mu, Yulian;Li, Hegang

作者机构：

关键词： I kappa B alpha gene;Porcine;Knockout;SSA;TALEN

期刊名称：INDIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：0.316； 五年影响因子：0.366 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Xenotransplantation often causes severe immunological rejection. The I kappa B alpha (inhibitor of nuclear factor kappa B alpha) gene plays an important role in delayed immunological rejection. Research shows that I kappa B alpha mutations can mitigate delayed immunological rejection. In the present study, the first exon sequence of the porcine I kappa B alpha gene was used to construct a transcription activator-like effector nuclease (TALEN) plasmid pair, and we used the double-strand break repair via single strand annealing (SSA) method to examine the knockout efficiency of the TALEN plasmid pair. The results showed that the knockout activity was 3.955, and the difference was extremely significant compared with the control group. A TALEN plasmid pair targeting the first exon sequence of the I kappa B alpha gene, was successfully constructed for the first time, and this study lays the foundation for knocking out the porcine I kappa B alpha gene to mitigate the delayed immunological rejection induced by xenotransplantation.

分类号： S8

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