Phenotype and Cellular Response of Wheat Lines Carrying Cre Genes to Heterodera avenae Pathotype Ha91

文献类型： 外文期刊

第一作者： Cui, Jiangkuan

作者： Cui, Jiangkuan;Peng, Huan;Huang, Wenkun;Liu, Shiming;Wu, Duqing;Kong, Lingan;He, Wenting;Peng, Deliang;Wu, Duqing

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期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

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收录情况： SCI

摘要： The cereal cyst nematode (CCN, Heterodera avenae), a major limiting factor for wheat production worldwide, is widespread in most wheat-growing regions in China. Accordingly, screening and characterization of resistant (R) wheat sources against H. avenae are very important. In this study, we screened 51 wheat lines, collected from the International Wheat and Maize Improvement Center (CIMMYT), carrying various Cre genes (Crel, Cre2, Cre3, Cre5, Cre7, Cre8, CreR, and Pi). From that screen, we identifiedone immune (M) culti var (with no adult females produced) and five resistant cul-tivars (with fewer than five females) to H. avenae pathotype Ha91. The Cre3 gene conferred the most effective resistance against H. avenae pathotype Ha91 in both field andgreenhouse assays. Conversely, the Crel and CreR genes conferred the poorest effective resistance. Using Pluronic F-127 gel and a staining assay, juvenile nematodes invading wheat roots were observed, and nematode development was analyzed. Compared withR and M roots, those of the susceptible (S) wheat cultivar Wenmail9 were more attractive to H. avenae second-stage juveniles (J2s). We observed the retardation of nematode development in R cultivars and tiny white female cysts protruding from the R cultivar VP1620. Nematodes in M roots either disintegrated or remained J2s or third-stage juveniles (J3s) and failed to complete their life cycle. Molting was also suppressed or delayed in R and M genotypes. For both S and R cultivars, syncytia were characterized by cell wall perforations and dense cytoplasm in hypertrophied syncytium component cells. Syncytial size increased gradually with nematode development in S cultivars. Moreover, an incompatibility reaction occurred in M wheat roots: the syncytium wasdisorganized, exhibiting disintegration and condensed nuclei. These sources of genetic resistance against CCN can potentially be planted in severely infested fields to reduce economic loss or can be used for introgression in breeding.

分类号： S4

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