Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a truncated version of -glucosidase from Aspergillus niger

文献类型： 外文期刊

第一作者： Li, Zhenjun

作者： Li, Zhenjun;Han, Hongjuan;Wang, Bo;Gao, Jianjie;Zhu, Bo;Peng, Rihe;Yao, Quanhong

作者机构：

关键词： 2-O--d-glucopyranosyl-l-ascorbic acid;-glucosidase;Aspergillus niger;high performance liquid chromatography;transglucosylation

期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）

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收录情况： SCI

摘要： A truncated version of -glucosidase gene (AglA) from Aspergillus niger, named as AglA-t in this study, was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that AglA was most close to the -glucosidase from Aureobasidium pullulans. In our research, we found that AglA-t could promote the formation of 2-O--d-glucopyranosyl-l-ascorbic acid (AA-2G) under optimized conditions. The major product was detected by high performance liquid chromatography, and confirmed to be by LC-MS analysis. The optimum pH and temperature for the transglucosylation reaction were 6.0 degrees C and 40 degrees C, respectively. The yield of AA-2G was significantly enhanced by metal ions Fe3+, Zn2+, Mg2+, and Al3+, and organic solvent isopropanol.

分类号： TS2

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