Cadmium Distribution and Characteristics of Cadmium-binding Proteins in Rice (Oryza sativa L.) Kernel

文献类型： 外文期刊

第一作者： Wei, Shuai

作者： Wei, Shuai;Guo, Boli;Jiang, Tao;Li, Ming;Wei, Yimin;Feng, Liuxing

作者机构：

关键词： cadmium distribution and speciation;rice kernel;Cd-binding proteins;LA-ICP-MS;SEC-ICP-MS

期刊名称：FOOD SCIENCE AND TECHNOLOGY RESEARCH （ 影响因子：0.668； 五年影响因子：0.895 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Studies were performed to investigate the Cadmium (Cd) distribution and Cd-binding proteins in rice kernel. Rice seeds [Oryza sativa L. (Akitakaomachi)] were cultured under Cd stress and after harvesting, the Cd distribution was determined using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The Cd concentrations and amino acid compositions in different storage protein fractions were analyzed using ICP-MS and an amino acid analyzer, respectively. The molecular weights of the Cd-binding proteins were determined by size exclusion chromatography-inductively coupled plasma mass spectrometry (SEC-ICP-MS). The Cd concentrations in rice kernel gradually decreased from the outer bran layers to the endosperm and Cd was particularly concentrated in the embryo, the outer pericarp and the aleurone layer. The Cd concentrations in different proteins fractions decreased in the following sequence: globulin > albumin > glutelin > prolamin. The presence of cysteine and methionine were closely related to the Cd concentration. The molecular weights of three prominent Cd-binding protein fractions were > 2000 k Da, 570 k Da and 131.9 k Da, respectively.

分类号： TS2

  • 相关文献
作者其他论文 更多>>

微信小程序