Differential gene expressions of NLRC5 signaling pathway in chicken macrophages cells response to Salmonella Enteritidis derived lipopplysaccharides stimulation

文献类型: 外文期刊

第一作者: Qiu, Lingling

作者: Qiu, Lingling;Chang, Guobin;Liu, Xiangping;Li, Zhiteng;Lu, Wei;Ren, Lichen;Xu, Lu;Wan, Fang;Guo, Xiaomin;Chen, Guohong;Qiu, Lingling;Chang, Guobin;Li, Zhiteng;Lu, Wei;Ren, Lichen;Xu, Lu;Wan, Fang;Guo, Xiaomin;Chen, Guohong

作者机构:

关键词: Chicken;Lipopplysaccharides;NLCR5;Signaling pathway

期刊名称:INDIAN JOURNAL OF ANIMAL RESEARCH ( 影响因子:0.44; 五年影响因子:0.543 )

ISSN:

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收录情况: SCI

摘要: As we all known, NLRC5 recognizes intracellular pathogen-associated molecular patterns (PAMPs) and provokes innate immune system. Its role in innate immune response, NF-kB activation and MHC-I expression remains controversial. In the present study, it was detected that differential gene expressions in NLRC5 signaling pathway at 2, 4, 6 and 8 hours after exposure to LPS using qRT-PCR technology, then analyzed its roles in host defense. The results showed that, comparing to control groups, the expression of NLRC5, MHC-I and IL-18 in LPS-treated groups were significantly up-regulated at 2 hours post stimulation (hps), TLR4 and NF-kB showed conspicuously up-regulated at 4 hps, while STAT1 was significantly down-regulated at 8 hps. Collectively, LPS did evoke inflammatory responses and NLRC5 may negatively regulate NF-kB and critically regulate MHC-I to control intracellular PAMPs in chicken macrophage cell line but the specific role of NLRC5 in host defense relates to cell types and species tested.

分类号: S8

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