Application of enzymatic probe sonication for selenium speciation in animal feeds

文献类型： 外文期刊

第一作者： Xiao, Zhiming

作者： Xiao, Zhiming;Rao, Zhenghua;Liu, Chengxin;Jia, Zheng;Suo, Decheng;Wang, Shi;Li, Yang;Fan, Xia;Li, Sheng;Guo, Jiangpeng

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关键词： Enzymatic probe sonication;Selenium speciation;Animal feeds;Atomic fluorescence spectrometry

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

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收录情况： SCI

摘要： Highlights?A novel enzymatic probe sonication based method to rapidly extraction of Se species from animal feeds is presented.?Method validation results demonstrated satisfactory analytical performance.?It was successfully applied for the analysis of Se species in real feed samples.?The results suggest that stricter measures should be taken to control the Se-enriched feed supplements in terms of Se species.AbstractEnzymatic probe sonication (EPS) was investigated as a novel and alternative technology for the extraction of five Se species SeCys2, MeSeCys, Se (IV), SeMet and Se (VI) from animal feeds. The critical parameters of EPS such as enzyme types, ultrasonic power, sample/enzyme ratio, extraction time and temperature were carefully optimized. Under current conditions, one sample can be extracted in 60s, in contrast to several hours required by the conventional enzymatic methods Moreover, the extraction is performed in pure water, no further temperature control or pH adjustment is needed, and therefore the risk of species interconvertion is drastically reduced or inhibited. The Se species were separated and quantitatively determined by high-performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS), which allows the separation of organic and inorganic Se species in a single chromatographic run. The newly developed method was successfully applied to the determination of Se species in 18 feed samples collected from markets and local farms. Concerning the feed samples studied, the results of this work suggest that stricter measures should be taken to control the Se-enriched feed supplements in terms of Se species.]]>

分类号： O65

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