Identification, characterization, and expression profiling of α-tocopherol biosynthesis genes associated with anilofos metabolism in Oryza sativa

文献类型： 外文期刊

第一作者： Wang, Xiao Lu

作者： Wang, Xiao Lu;He, Zhi Jiang;Zeng, Li Qing;Zeng, Ying Yu;Zhou, Zhi Zhong;Liu, Xiao Liang;Cheng, Xi Ran;Chen, Zhao Jie;Chen, He

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关键词： alpha-tocopherol synthetase; Anilofos; Rice; Metabolism; Bioinformatics analysis

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

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年卷期： 2025 年 12 卷 1 期

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收录情况： SCI

摘要： BackgroundVitamin E, particularly alpha-tocopherol (alpha-TOC), is a chloroplast-synthesized antioxidant that plays a crucial role in protecting photosynthetic membranes from oxidative damage caused by environmental stressors. Its biosynthesis involves several key enzymes: hydroxyphenylpyruvate dioxygenase (HPPD), homogentisate phytyltransferase (HPT), tocopherol cyclase (VTE1), tocopherol methyltransferase (VTE4), and phytolkinase (VTE5). However, the expression patterns and functional roles of genes involved in alpha-TOC biosynthesis under pesticide stress remain poorly characterized.ResultsIn this study, we identified 10 alpha-TOC biosynthesis genes, including 2 HPPD, 1 HPT, 2 VTE1, 1 VTE4, and 4 VTE5 genes, in rice (Oryza sativa) genome using transcriptome datasets from plants treated with anilofos and alpha-TOC to explore their properties under pesticide stress. Phylogenetic analysis classified the alpha-TOC biosynthesis gene family into five subfamilies across rice, Arabidopsis (Arabidopsis thaliana), soybean (Glycine max), wheat (Triticum aestivum), and maize (Zea mays). Chromosomal localization revealed that segmental duplications contributed to the expansion of this gene family in rice, with 10 genes distributed across 7 of its 12 chromosomes. In addition, 10 rice genes involved in alpha-TOC biosynthesis exhibited collinearity with corresponding genes in Arabidopsis, soybean, wheat, and maize. In silico subcellular localization prediction suggested that the proteins encoded by these 10 genes localize to multiple cellular compartments, including the nucleus, cytoplasm, mitochondria, plasma membrane, and endoplasmic reticulum. OsHPPD, OsHPT, OsVTE1, OsVTE4, and OsVTE5 possess diverse gene structures, cis-regulatory elements, motifs, and conserved domains, suggesting their diverse functions in response to biotic and abiotic stresses. RT-qPCR analysis confirmed that a subset of these genes was preferentially expressed under pesticide exposure. Notably, combined treatment with 250 mg/L alpha-TOC and 0.04 mg/L anilofos upregulated the expression of all 10 genes by 1.64-3.75-fold in roots and by 2.66-5.34-fold in shoots compared with anilofos-only treatment. Protein-protein interaction analysis further supported the involvement of these 10 rice alpha-TOC biosynthesis proteins in anilofos metabolism.ConclusionsThese findings indicate that alpha-TOC biosynthesis genes respond effectively to anilofos-induced stress, likely facilitating alpha-TOC production and potentially regulating pesticide degradation.

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