Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method
文献类型: 外文期刊
第一作者: Zhang, Yongchun
作者: Zhang, Yongchun;Zhao, Xiao;Shi, Bin;Cai, Youming;Zhao, Kai;Zhao, Xiao;Zhao, Kai;Xu, Yan;Fan, Xiaorui;Du, Yanan;Zhang, Qi;Zhang, Xiaoxia;Zhao, Binan;Yang, Dan;Liang, Jieling;Zhu, Ziping
作者机构:
关键词: LAMP;ornamental plant;Potyvirus;virus detection
期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )
ISSN: 0304-8608
年卷期: 2018 年 163 卷 2 期
页码:
收录情况: SCI
摘要: Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
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