Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method

文献类型: 外文期刊

第一作者: Zhang, Yongchun

作者: Zhang, Yongchun;Zhao, Xiao;Shi, Bin;Cai, Youming;Zhao, Kai;Zhao, Xiao;Zhao, Kai;Xu, Yan;Fan, Xiaorui;Du, Yanan;Zhang, Qi;Zhang, Xiaoxia;Zhao, Binan;Yang, Dan;Liang, Jieling;Zhu, Ziping

作者机构:

关键词: LAMP;ornamental plant;Potyvirus;virus detection

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

ISSN: 0304-8608

年卷期: 2018 年 163 卷 2 期

页码:

收录情况: SCI

摘要: Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

分类号:

  • 相关文献

[1]Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of Batai Virus in Cattle and Mosquitoes. Liu, Hao,Li, Xin Tong,Hu, Bo,Zhang, Lei,Xue, Xiang-hong,Lv, Shuang,Lu, Rong-guang,Shi, Ning,Yan, Xi-jun. 2016

[2]Influence of Nutrient Formula, Concentration and pH on the Growth of Dracaena cambodiana Hydrocultured in Glass Container and Optimizing for Nutrition Supplement. Wang, Dairong,Liu, Fei,Liao, Feixiong,Liu, Xiaorong. 2013

[3]Nineteenth century French rose Rosa sp.) germplasm shows a shift over time from a European to an Asian genetic background. Liorzou, Mathilde,Pernet, Alix,Li, Shubin,Chastellier, Annie,Thouroude, Tatiana,Michel, Gilles,Malecot, Valery,Gaillard, Sylvain,Foucher, Fabrice,Clotault, Jeremy,Grapin, Agnes,Li, Shubin,Briee, Celine,Oghina-Pavie, Cristiana.

[4]Using Small RNA-seq Data to Detect siRNA Duplexes Induced by Plant Viruses. Niu, Xiaoran,Sun, Yu,Gao, Shan,Niu, Xiaoran,Chen, Ze,Niu, Xiaoran,Chen, Ze,Li, Rugang,Padmanabhan, Chellappan,Ling, KaiShu,Ruan, Jishou,Kreuze, Jan F.,Fei, ZhangJun. 2017

[5]Selection of an aptamer against Muscovy duck parvovirus for highly sensitive rapid visual detection by label-free aptasensor. Lu, Taofeng,Ma, Qin,Yan, Wenzhuo,Wang, Yuanzhi,Zhang, Yuanyuan,Zhao, Lili,Chen, Hongyan. 2018

[6]Rapid and sensitive detection of Banana bunchy top virus by loop-mediated isothermal amplification. Peng, Jun,Xia, Zihao,Li, Yongqiang,Fan, Zaifeng,Peng, Jun,Xia, Zihao,Li, Yongqiang,Fan, Zaifeng,Peng, Jun,Zhang, Junfang,Huang, Junsheng. 2012

[7]Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallen). Xu, Qiufang,Liu, Haoqiu,Yuan, Pingping,Zhang, Xiaoxia,Chen, Qingqing,Zhou, Yijun,Yuan, Pingping,Jiang, Xuanli. 2017

[8]The Effect of Major Viruses and Virus-free Planting Materials on Sweetpotato Root Yield in China. Zhang Li-ming,Wang Qing-mei,Ma Dai-fu,Wang Yi.

[9]Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China. Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui,Chen, Jia,Shao, Yun-Hua,Li, Xiang-Dong,Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui. 2009

[10]RNAi-mediated SMV P3 cistron silencing confers significantly enhanced resistance to multiple Potyvirus strains and isolates in transgenic soybean. Yang, Xiangdong,Niu, Lu,Zhang, Wei,Yang, Jing,Xing, Guojie,He, Hongli,Guo, Dongquan,Du, Qian,Qian, Xueyan,Yao, Yao,Li, Qiyun,Dong, Yingshan. 2018

[11]A NEW ISOLATE OF CHILLI VEINAL MOTTLE VIRUS THAT INFECTS TOBACCO IN CHINA. Yang, J.,Wang, R.,Luo, Z. P.,Dong, J. H.,Zhang, T. J.,Zhang, Z. K.,Luo, H. Y.. 2013

[12]The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Cui, Xiaoyan,Wu, Guanwei,Chen, Xin,Cui, Xiaoyan,Yaghmaiean, Hoda,Wu, Guanwei,Wu, Xiaoyan,Wang, Aiming,Cui, Xiaoyan,Yaghmaiean, Hoda,Wu, Guanwei,Thorn, Greg,Wang, Aiming,Wu, Xiaoyan,Yaghmaiean, Hoda. 2017

[13]Protein-protein interactions in two potyviruses using the yeast two-hybrid system. Lin, Lin,Shi, Yuhong,Luo, Zhaopeng,Lu, Yuwen,Zheng, Hongying,Yan, Fei,Chen, Jiong,Chen, Jianping,Lin, Lin,Wu, Yunfeng,Adams, M. J.. 2009

[14]Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system. Shen, W. T.,Wang, M. Q.,Yan, P.,Gao, L.,Zhou, P.. 2010

[15]Identification of differentially regulated maize proteins conditioning Sugarcane mosaic virus systemic infection. Chen, Hui,Xia, Zihao,Xie, Jipeng,Wu, Boming,Fan, Zaifeng,Zhou, Tao,Chen, Hui,Xia, Zihao,Xie, Jipeng,Wu, Boming,Fan, Zaifeng,Zhou, Tao,Cao, Yanyong,Li, Yiqing,Carr, John P..

[16]NbEXPA1, an alpha-expansin, is plasmodesmata-specific and a novel host factor for potyviral infection. Li, Fangfang,Renaud, Justin,Shen, Wentao,Li, Yinzi,Guo, Lihua,Cui, Hongguang,Sumarah, Mark,Wang, Aiming,Park, Sang-Ho,Li, Yinzi,Wang, Aiming,Shen, Wentao,Guo, Lihua,Park, Sang-Ho,Cui, Hongguang. 2017

[17]A fitness cost for Turnip mosaic virus to overcome host resistance. Jenner, CE,Wang, XW,Ponz, F,Walsh, JA. 2002

[18]Interaction between potyvirus P3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants. Lin, Lin,Yan, Fei,Lu, Yuwen,Zheng, Hongying,Chen, Jianping,Luo, Zhaopeng. 2011

[19]Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus. Gong, Dian,Wang, Jian-Hua,Lin, Zhan-Song,Zhang, Shao-Yan,Zhang, Yu-Liang,Yu, Nai-Tong,Liu, Zhi-Xin,Gong, Dian,Lin, Zhan-Song,Zhang, Shao-Yan,Yu, Nai-Tong,Xiong, Zhongguo,Xiong, Zhongguo. 2011

[20]Gain of virulence by Soybean mosaic virus on Rsv4-genotype soybeans is associated with a relative fitness loss in a susceptible host. Wang, Y.,Hajimorad, M. R.,Wang, Y..

作者其他论文 更多>>