Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris
文献类型: 外文期刊
第一作者: Wang, Bo
作者: Wang, Bo;Wang, Xiuying;Tian, Yongsheng;Li, Zhenjun;Gao, Jianjie;Peng, Rihe;Yao, Quanhong;Wang, Xiuying;Yan, Ying
作者机构:
关键词: Laccaria bicolor;laccase;Pichia pastoris;synthetic dyes
期刊名称:BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT ( 影响因子:1.632; 五年影响因子:2.029 )
ISSN: 1310-2818
年卷期: 2016 年 30 卷 1 期
页码:
收录情况: SCI
摘要: Synthetic dyes are known to be highly toxic to mammalian cells and mutagenic and carcinogenic to humans and, therefore, should be detoxified and removed from industrial effluents. Different approaches for removal and detoxication are extensively sought. Biochemical methods are considered the most economical and effective method of dye decolourization. In this research, the laccase gene from Laccaria bicolor was modified and expressed in Pichia pastoris. The properties of the recombinant laccase and its ability to degrade synthetic dyes were studied. The laccase activity was optimal at pH 2.2 and 50 degrees C. Its K-m value was 0.187 mmol/L for ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)]. The laccase obtained was shown to decolorize the synthetic dyes, malachite green, crystal violet and orange G, with ABTS as a mediator. These results indicated that the laccase obtained may be used to treat industrial effluents containing artificial dyes.
分类号:
- 相关文献
作者其他论文 更多>>
-
Whole-Genome Sequencing of Flammulina filiformis and Multi-Omics Analysis in Response to Low Temperature
作者:Liang, Xinmin;Han, Jing;Shu, Xueqin;Wang, Bo;Jia, Dinghong;Peng, Weihong;He, Xiaolan;Liu, Xun;Cui, Yuqin;Peng, Weihong;Lei, Mengting
关键词:
Flammulina filiformis ; whole-genome sequencing; transcriptome; metabolome; cold adaptation -
Adsorption and in vitro controlled-release properties of a soybean cellulose nanocrystal/polyacrylamide-based hydrogel as a carrier for different polyphenols
作者:Tang, Lu;Zhao, Dan;Wang, Bo;Bai, Shiru;Fan, Bei;Zhang, Liang;Wang, Fengzhong;Wang, Bo
关键词:Hydrogel; Polyphenols; Absorption; Release; Bioavailability
-
Genome-Wide Identification and Comprehensive Analysis of the PPO Gene Family in Glycine max and Glycine soja
作者:Song, Ziye;Dong, Yingshan;Song, Ziye;Wang, Bo;Liu, Jia;Liu, Nianxi;Yi, Zhigang;Li, Zhi;Dong, Zhimin;Zhang, Chunbao;Dong, Yingshan;Li, Yuqiu
关键词:
Glycine max ;Glycine soja ;PPO ; gene family -
Enhanced healing of MRSA-infected wounds with okara cellulose nanocrystals-based temperature-sensitive cationic hydrogel: Development and characterization
作者:Tang, Lu;Wang, Bo;Bai, Shiru;Liu, Xiaolin;Zhao, Dan;Fan, Bei;Zhang, Liang;Wang, Fengzhong;Wang, Bo;Wang, Fengzhong;Wang, Fengzhong
关键词:Hydrogel; Functional properties; MRSA-infected wound healing
-
OsPRMT5 methylates OsPAL1 to promote rice resistance, hindered by a Xanthomonas oryzae effector
作者:Sheng, Cong;Wang, Bo;Chen, Wenchan;Guo, Baodian;Zhao, Yancun;Liu, Fengquan;Li, Kaihuai;Liu, Fengquan;Wang, Bo;Chen, Wenchan;Qiao, Lulu;Zhao, Hongwei
关键词:effector; OsPAL1; OsPRMT5; protein arginine methylation; rice bacterial blight; SA
-
Octenyl succinic anhydride starch enhanced 3D printability of corn starch-based emulsion-filled gels incorporating egg yolk
作者:Zhong, Yuanliang;Lv, Weiqiao;Xiao, Hongwei;Wang, Bo;Li, Bingzheng;Zhao, Dan
关键词:3D printing; Octenyl succinic anhydride; Emulsion gel; Egg yolk; Starch
-
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion
作者:Zhao, Lanmei;Sun, Mengxue;Meng, Long;Wang, Bo;Lyu, Can;Liu, Jian;Liu, Jian
关键词:PHA production; Biofilm; Bioconversion; Extracellular polymeric substances; Functional microorganisms; Network connections