A new rice zinc-finger protein binds to the O2S box of the alpha-amylase gene promoter

文献类型： 外文期刊

第一作者： Peng, RH

作者： Peng, RH;Yao, QH;Xiong, AS;Fan, HQ;Li, X;Peng, YL;Cheng, ZM;Li, Y

作者机构：

关键词： rice zinc-induced protein;O2S box;yeast one-hybrid system

期刊名称：EUROPEAN JOURNAL OF BIOCHEMISTRY （ 影响因子：3.579； ）

ISSN： 0014-2956

年卷期： 2004 年 271 卷 14 期

页码：

收录情况： SCI

摘要： A putative transcription factor, named RAMY, that binds to the 20-bp O2S sequences of the regulatory region of the Amy2 gene promoter has been identified using the yeast one-hybrid system from a rice library. The full length RAMY cDNA clone encodes a 218-amino acid protein and is homologous to the late embryogenesis-abundant protein (LEA5). In vitro mutagenesis and electrophoretic mobility shift assays confirmed that RAMY can bind with O2S specifically through an unusual zinc finger with a CXCX4CX2H consensus sequence. Low levels of RAMY mRNAs were detected in rice leaves and roots by Northern blot hybridization. The plant hormone gibberellin (GA) induces expression of both RAMY and Amy2 genes, as performed by Northern blot hybridization, but the increase in RAMY mRNA level occurs prior to that of the Amy2 mRNA level in the GA-treated aleurone tissues. These data suggest that RAMY may act as a trans-acting protein and is probably involved in the GA-induced expression of the rice alpha-amylase gene.

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