Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum

文献类型： 外文期刊

第一作者： Zhang, JS

作者： Zhang, JS;Tang, QJ;Zimmerman-Kordmann, M;Reutter, W;Fan, H

作者机构：

关键词： Ganoderma lucidum (GL);immunoactivation;B cells;proteoglycan

期刊名称：LIFE SCIENCES （ 影响因子：5.037； 五年影响因子：4.689 ）

ISSN： 0024-3205

年卷期： 2002 年 71 卷 6 期

页码：

收录情况： SCI

摘要： A bioactive fraction (GLIS) was isolated from the fruiting body of the fungus Ganoderma lucidum using successive chromatographic steps. GLIS is a proteoglycan and has a carbohydrate: protein ratio of 11.5 : 1. The carbohydrate portion is composed of seven different monosaccharides, predominantly D-glucose, D-galactose and D-mannose in the molar ratio of 3.0 : 1 : 1. GLIS stimulated the proliferation of mouse spleen lymphocytes, resulting in a three to four-fold increase in the percentage of B cells. GLIS also activated mouse spleen lymphocytes, and most of the activated cells were B cells. The B cells were enlarged, expressed CD71 and CD25 on the cell surface, and showed an increase in the secretion of immunoglobulin. Lymphocytes also showed a slightly increased production of IL-2, whereas the secretion of IL-4 was not influenced by GLIS. Furthermore, GLIS did not influence the intracellular Ca2+ concentration of lymphocytes, but it enhanced the expression of protein kinase C alpha and protein kinase C gamma in B cells. According to our results GLIS is a new B cell-stimulating factor. (C) 2002 Elsevier Science Inc. All rights reserved.

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