Cloning and characterization of the Na+/H+ antiport genes from Triticum aestivum

文献类型： 外文期刊

第一作者： Wang, ZN

作者： Wang, ZN;Zhang, JS;Guo, BH;He, SJ;Tian, AG;Chen, SY

作者机构：

关键词： wheat;Na+/H+ antiport gene;salt-inducible

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2002 年 44 卷 10 期

页码：

收录情况： SCI

摘要： The Na+/H+ antiport genes named TaNHX1 and TaNHX2 were cloned by screening a salt-stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed that TaNHX1 was 2 029 by in length and contained a complete ORF of 1 638 by. The TaNHX1 encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI. TaNHX2 was 1 693 by in length consisting of a partial ORF followed by a 3'-UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. A RT-PCR assay showed that the level of TaNHX1 transcripts was increased and. reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.

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