Cryopreservation of apple dormant buds and shoot tips
文献类型: 外文期刊
第一作者: Wu, YJ
作者: Wu, YJ;Zhao, YH;Engelmann, F;Zhou, MD;Zhang, DM;Chen, SY
作者机构:
关键词: Malus domestica;dormant buds;shoot tips;cryopreservation
期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )
ISSN: 0143-2044
年卷期: 2001 年 22 卷 6 期
页码:
收录情况: SCI
摘要: Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds immediately before freezing were successfully cryopreserved using three different methods (vitrification, encapsulation-dehydration and two-step freezing). Two-step freezing produced the highest in vitro growth recovery (83%) of cryopreserved shoot tips, followed by vitrification (60%) and encapsulation-dehydration (33%). By contrast, when dormant buds were cryopreserved using the two-step freezing technique, shoot tips excised from buds after rewarming and cultured in vitro displayed only 16% recovery.
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