Cryopreservation of apple dormant buds and shoot tips

文献类型: 外文期刊

第一作者: Wu, YJ

作者: Wu, YJ;Zhao, YH;Engelmann, F;Zhou, MD;Zhang, DM;Chen, SY

作者机构:

关键词: Malus domestica;dormant buds;shoot tips;cryopreservation

期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )

ISSN: 0143-2044

年卷期: 2001 年 22 卷 6 期

页码:

收录情况: SCI

摘要: Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds immediately before freezing were successfully cryopreserved using three different methods (vitrification, encapsulation-dehydration and two-step freezing). Two-step freezing produced the highest in vitro growth recovery (83%) of cryopreserved shoot tips, followed by vitrification (60%) and encapsulation-dehydration (33%). By contrast, when dormant buds were cryopreserved using the two-step freezing technique, shoot tips excised from buds after rewarming and cultured in vitro displayed only 16% recovery.

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