Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH)

文献类型： 外文期刊

第一作者： Guo, BH

作者： Guo, BH;Zhang, YM;Li, HJ;Du, LQ;Li, YX;Zhang, JS;Chen, SY;Zhu, ZQ

作者机构：

关键词： BADH gene;transformation;microprojectile bombardment;wheat

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2000 年 42 卷 3 期

页码：

收录情况： SCI

摘要： Betaine aldehyde dehydrogenase (BADH) cDNA cloned from Atriplex hortensis L. in the plasmid pABH9 containing maize ubiquitin promoter and bar gene was transferred into wheat (Triticum aestivum L.) by microprojectile bombardment with 4.1% of average frequency of transformation. From 300 young embryo calli bombarded with the plasmid, 24 transgenic plants were obtained showing BADH gene integration by both PCR and Southern blotting analysis. Among the 24 transgenic plants, 13 exhibited higher BADH activity than the control. Some transgenic plants grew normally with healthy roots on the medium containing 0.7% NaCl while the control plants had very poor roots and finally died.

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