Histone deacetylase inhibitor stimulates E2 and P4 secretion in sika deer ovarian granulosa cells at a moderate dose

文献类型： 外文期刊

第一作者： Xing, Mingjie

作者： Xing, Mingjie;Chen, Xiumin;Li, Xiaoxia;Wang, Xiaoxu;Cao, Xinyan;Cui, Xuezhe;Chang, Tong;Xu, Baozeng;Wei, Haijun;Yang, Yifeng;Xue, Hailong;Wang, Shiyong;Diao, Yunfei;Zhao, Weigang;Zhao, Meng

作者机构：

关键词： apoptosis;cell cycle;granulosa cells;histone acetylation;TSA

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

ISSN： 1065-6995

年卷期： 2018 年 42 卷 3 期

页码：

收录情况： SCI

摘要： The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4. TSA pretreatment increased DNMT3a, DNMT1, HDAC1, and HAT1 expression, and attenuated them when TAS higher than 50nM. The protein levels of H3K9ac and H4K8ac in GCs were increased at 48h after TSA treatment. TSA stimulated the secretion of estradiol and progesterone at a moderate dose. Our data suggest that TSA is important as a regulator of steroid hormone synthesis in granulosa cells during follicular development in the sika deer ovary.

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