Elimination of Grapevine rupestris stem pitting-associated virus from Vitis vinifera 'Kyoho' by an antiviral agent combined with shoot tip culture

文献类型： 外文期刊

第一作者： Hu, Guojun

作者： Hu, Guojun;Dong, Yafeng;Zhang, Zunping;Fan, Xudong;Ren, Fang;Li, Zhengnan;Zhang, Shuangna

作者机构：

关键词： Grapevine rupestris stem pitting-associated virus (GRSPaV);In vitro-cultured grapevine;Chemotherapy;Shoot regeneration;Virus elimination

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2018 年 229 卷

页码：

收录情况： SCI

摘要： We evaluated the ability of different concentrations of Ribavirin (15 and 25 mu g/mL, R15 and R25) to eliminate Grapevine rupestris stem pitting-associated virus (GRSPaV) from in vitro-cultured grapevine plants. Vitis vinifera 'Kyoho' plants infected by GRSPaV were used as the original plant materials. All of the plants survived in the treatment with R15 and R25 for 85 days, but all Ribavirin-treated plants exhibited phytotoxicity symptoms after 25 days. To ensure the accuracy of detection, optimal primer pair combinations (cpFlFt2-cpF2R1 and replF1R2-replF2R1) were used to assess the eradication efficiency of GRSPaV from regenerated grapevine plants with a nested reverse transcription polymerase chain reaction. The results showed that the efficiency of the GRSPaV eradication was associated to virus titer of regenerated plant in different detection period. All surviving regenerated plants were tested for two rounds and the average elimination rates of GRSPaV in the two periods showed more different, 74.8% (113/151) and 52.3% (79/151), respectively. In addition, the efficiency of the GRSPaV eradication also depended on the concentrations and treatment duration of Ribavirin. The average elimination rates for the regenerated R25-treated and R15-treated plants in the second round detection were 54.1 and 50.6%, respectively. Moreover, the elimination rates for the R25-treated plants were 21.4, 37.5, and 77.8% after 45, 65, and 85 days of treatment, respectively. Combination of shoot tip culture with chemotherapy may greatly increase the efficiency of viral eradications.

分类号：

  • 相关文献

[1]Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Hu, Guojun,Dong, Yafeng,Zhang, Zunping,Fan, Xudong,Ren, Fang,Zhou, Jun.

[2]Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy. Hu, G. J.,Hong, N.,Wang, L. P.,Wang, G. P.,Hong, N.,Wang, G. P.,Hu, H. J.. 2012

[3]Hydrothermal synthesis of titanium dioxide nanoparticles: mosquitocidal potential and anticancer activity on human breast cancer cells (MCF-7). Murugan, Kadarkarai,Dinesh, Devakumar,Paulpandi, Manickam,Ponraj, Thondhi,Subramaniam, Jayapal,Rajaganesh, Rajapandian,Kavithaa, Krishnamoorthy,Alsalhi, Mohamad Saleh,Devanesan, Sandhanasamy,Wei, Hui,Kumar, Suresh,Nicoletti, Marcello,Benelli, Giovanni.

[4]Efficacy of virus elimination from apple by thermotherapy coupled with in vivo shoot-tip grafting and in vitro meristem culture. Hu, Guo-jun,Dong, Ya-feng,Zhang, Zun-ping,Fan, Xu-dong,Ren, Fang,Li, Zheng-nan.

[5]Validated hydrophilic interaction LC-MS/MS method for simultaneous quantification of dacarbazine and 5-amino-4-imidazole-carboxamide in human plasma. Yang, Yuhui,Zhang, Weihua,Liu, Yanhong.

[6]Efficiency of virus elimination from potted apple plants by thermotherapy coupled with shoot-tip grafting. Hu, G. -J.,Zhang, Z. -P.,Dong, Y. -F.,Fan, X. -D.,Ren, F.,Zhu, H. -J..

[7]Vitamin B(6) Points PC6 Injection During Acupuncture Can Relieve Nausea and Vomiting in Patients With Ovarian Cancer. You, Qi,Yu, Hongli,Wu, Donglai,Zhang, Yuanlong,Zheng, Jianhua,Peng, Chunjie. 2009

[8]Regeneration of transgenic plants from cell suspensions of transformed apple (Malus X domestica Borkh. cv. Gala). Wu, Yongjie,Li, Yusheng,Wu, Yaqin,Cheng, Hehe,Zhao, Yanhua,Li, Yunhe,Li, Yin.

[9]Plant regeneration capacity of mesophyll protoplasts from Brassica napus and related species. Hu, Q,Andersen, SB,Hansen, LN. 1999

[10]An efficient shoot regeneration system and Agrobacterium-mediated transformation with codA gene in a doubled haploid line of broccoli. Sheng, Xiaoguang,Gu, Honghui,Yu, Huifang,Wang, Jiansheng,Zhao, Zhenqing,Qi, Zirong. 2016

[11]Culture of shoot tips from adventitious shoots can eradicate Apple stem pitting virus but fails in Apple stem grooving virus. Wang, Min-Rui,Li, Bai-Quan,Feng, Chao-Hong,Wang, Qiao-Chun,Feng, Chao-Hong.

[12]Variation of phenotype, ploidy level, and organogenic potential of in vitro regenerated polyploids of Pyrus communis. Sun, Qingrong,Sun, Hongyan,Li, Huifeng,Xin, Li,Bell, Richard L..

[13]Micropropagation and Adventitious Shoot Regeneration of Healthy and Phytoplasma-Infected Jujube. Sun, Q.,Sun, H.,Li, G.,Shen, G. N.,Zhou, G. F..

[14]Genetic transformation of the sugar beet plastome. De Marchis, Francesca,Wang, Yongxin,Stevanato, Piergiorgio,Arcioni, Sergio,Bellucci, Michele. 2009

[15]Comparative Organogenic Response of Six Clonal Apple Rootstock Cultivars. Sun, Qingrong,Sun, Hongyan,Li, Linguang,Tao, Jihan,Sun, Meijuan,Bell, Richard L.,Zhang, Wei.

作者其他论文 更多>>

微信小程序