Application of high-speed counter-current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum
文献类型: 外文期刊
第一作者: Chen, Bao
作者: Chen, Bao;Liu, Zhengbo;Zhang, Yuwei;Wang, Yufang;Wang, Yanhua;Sun, Yinshi;Chen, Bao;Li, Wei;Wang, Yanhua;Sun, Yanjun
作者机构:
关键词: high-speed counter-current chromatography;lobetyolin;platetyolin;polyacetylenes;traditional Chinese medicine
期刊名称:JOURNAL OF SEPARATION SCIENCE ( 影响因子:3.645; 五年影响因子:2.943 )
ISSN: 1615-9306
年卷期: 2018 年 41 卷 3 期
页码:
收录情况: SCI
摘要: Three polyacetylenes were isolated and purified from Platycodon grandiflorum A. DC for the first time by high-speed counter-current chromatography using a two-phase solvent system composed of hexane/ethyl acetate/methanol/water (1:31:1:31, v/v/v/v) and high-performance liquid chromatography with an Agilent ZORBAX (R) SB-C18 column (4.6 mm x 150 mm, 5 mu m). After separation by high-speed counter-current chromatography and high-performance liquid chromatography, we obtained 3.5 mg of platetyolin A, 4.1 mg of platetyolin B, and 18.1 mg of lobetyolin with purities of 97.2, 96.7, and 96.9%, respectively. The purity of each compound was assessed by high-performance liquid chromatography and the chemical structures were evaluated by high-resolution electrospray ionization time-of-flight mass spectrometry and oneand two-dimensional NMR spectroscopy. Among the isolated compounds, platetyolin A and platetyolin B are newly reported compounds.
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