Gene screening and differential expression analysis of microRNAs in the middle silk gland of wild-type and naked pupa mutant silkworms (Bombyx mori)

文献类型： 外文期刊

第一作者： Qian, Ping

作者： Qian, Ping;Wang, Xin;Jiang, Tao;Song, Fei;Chen, Chen;Fan, Yangyang;Shen, Xingjia;Qian, Ping;Shen, Xingjia

作者机构：

关键词： Bombyx mori;MicroRNA;Middle silk gland;Naked pupa mutant

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2016 年 19 卷 2 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are a class of endogenous, non-coding small RNAs that serve as important post transcriptional gene expression regulators and play important roles in the silkworm (Bombyx mori) development, growth, and viral immunity. However, information on the diversity of these regulatory RNAs in the middle silk gland (MSG) of naked pupa (Nd) mutant silkworms is limited. In this study, by using Solexa high-throughput sequencing technology, we identified and compared small RNA libraries from the MSG of wild-type silkworm P50(MSG-P50) and the Nd mutant (MSG-Nd), respectively. A total of 272 conserved and 333 novel miRNAs were identified, in which 141 ones showed significantly different expression patterns between MSG-P50 and MSG-Nd, and 10 ones were randomly selected and validated by stem-loop quantitative reverse-transcription polymerase chain reaction (qRT-PCR). In addition, potential targets were predicted for differentially expressed miRNAs based on sequence complementation between miRNAs and their target genes. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed miRNAs that actively participate in various life processes and three pathways associated with protein synthesis including endoplasmic reticulum pathway, ribosome pathway, and ribosome biogenesis in eukaryotes, were significantly disrupted in MSG-Nd. This is the first comprehensive description of miRNAs in the silkworm MSG. Overall, the results provide useful information for future studies on miRNAs and suggest that the fibroin synthetic deficiency in the posterior silk gland impairs the sericin secretion process in MSG. (C) 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

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