Truncation of C-terminal 20 amino acids in PA-X contributes to adaptation of swine influenza virus in pigs

文献类型： 外文期刊

第一作者： Xu, Guanlong

作者： Xu, Guanlong;Zhang, Xuxiao;Sun, Yipeng;Sun, Honglei;Xiong, Xin;Jiang, Ming;He, Qiming;Wang, Yu;Pu, Juan;Guo, Xin;Yang, Hanchun;Liu, Jinhua;Xu, Guanlong;Zhang, Xuxiao;Sun, Yipeng;Sun, Honglei;Xiong, Xin;Jiang, Ming;He, Qiming;Wang, Yu;Pu, Juan;Guo, Xin;Yang, Hanchun;Liu, Jinhua;Liu, Qinfang

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： The PA-X protein is a fusion protein incorporating the N-terminal 191 amino acids of the PA protein with a short C-terminal sequence encoded by an overlapping ORF (X-ORF) in segment 3 that is accessed by + 1 ribosomal frameshifting, and this X-ORF exists in either full length or a truncated form (either 61-or 41-condons). Genetic evolution analysis indicates that all swine influenza viruses (SIVs) possessed full-length PA-X prior to 1985, but since then SIVs with truncated PA-X have gradually increased and become dominant, implying that truncation of this protein may contribute to the adaptation of influenza virus in pigs. To verify this hypothesis, we constructed PA-X extended viruses in the background of a "triple-reassortment" H1N2 SIV with truncated PA-X, and evaluated their biological characteristics in vitro and in vivo. Compared with full-length PA-X, SIV with truncated PA-X had increased viral replication in porcine cells and swine respiratory tissues, along with enhanced pathogenicity, replication and transmissibility in pigs. Furthermore, we found that truncation of PA-X improved the inhibition of IFN-I mRNA expression. Hereby, our results imply that truncation of PA-X may contribute to the adaptation of SIV in pigs.

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