Transcriptomic analysis of porcine PBMCs infected with Toxoplasma gondii RH strain

文献类型： 外文期刊

第一作者： Zhou, Chun-Xue

作者： Zhou, Chun-Xue;Zhou, Dong-Hui;Liu, Guang-Xue;Zhu, Xing-Quan;Zhou, Chun-Xue;Suo, Xun;Zhou, Chun-Xue;Suo, Xun

作者机构：

关键词： Toxoplasma gondii;Pig peripheral blood mononuclear cells;Transcriptomic analysis

期刊名称：ACTA TROPICA （ 影响因子：3.112； 五年影响因子：3.002 ）

ISSN： 0001-706X

年卷期： 2016 年 154 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondii is an obligate, intracellular protozoan that can infect virtually all warm-blooded animals. Humans can be infected through the consumption of undercooked meat of farm animals. However, there are few reports on pathogenic mechanism and immune regulation during the interaction between T. gondii and domesticated animals. RNA extracted from pig peripheral blood mononuclear cells (PBMCs) infected with T. gondii RH strain at different time points (0 h, 8 h, 24 h, and 48 h) were reverse transcribed into cDNA and sequenced using Illumina technology. After ruling out short-length and low-quality sequences, more than 18 million clean reads and 14 million unique reads were acquired in each group. Clean reads were then mapped to the pig reference genome and differently expressed (DE) genes were identified. DE genes increased along with the extension of infection time. For function classification and pathway analysis, annotated DE genes were categorized into three Gene Ontology (GO) categories and assigned to six major Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, 10 DE genes were identified to be involved in some important immune processes and signaling pathways at the early infection stage. This is the first transcriptomic analysis of pig PBMCs infected by T. gondii. Functional DE genes and KEGG pathways indentified at different time points in this study will help to better understand the host immunity and defense mechanisms and promote the prevention and control of T. gondii infection. (C) 2015 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Transcriptomic analysis of porcine PBMCs in response to FMDV infection. Zhao, Fu-Rong,Xie, Yin-Li,Zhang, Yong-Guang,Chang, Hui-Yun,Zhang, Yong-Guang,Chang, Hui-Yun. 2017

[2]Identification and expression pattern of candidate olfactory genes in Chrysoperla sinica by antennal transcriptome analysis. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Si-BaoWang,Chun-Yi Wang,Li-Min Lv,Shuang-Lin Dong,Jin-Jie Cui.

[3]Morphological Structure and Transcriptome Comparison of the Cytoplasmic Male Sterility Line in Brassica napus (SaNa-1A) Derived from Somatic Hybridization and Its Maintainer Line SaNa-1B. Du, Kun,Liu, Qier,Wu, Xinyue,Jiang, Jinjin,Wu, Jian,Fang, Yujie,Wang, Youping,Li, Aimin. 2016

[4]Transcriptomic analyses reveal complex and interconnected sucrose signaling cascades in developing seeds of castor bean. Wang, Bin,Haque, Mohammad Enamul,Xu, Wei,Li, Fei,Liu, Aizhong,Wang, Bin,Haque, Mohammad Enamul,Zhang, Yang,Liu, Aizhong. 2018

[5]Identification of regulatory networks and hub genes controlling soybean seed set and size using RNA sequencing analysis. Du, Juan,Wang, Shoudong,He, Cunman,Shou, Huixia,Zhou, Bin,Ruan, Yong-Ling.

[6]De novo transcriptomic analysis and development of EST-SSR markers in the Siberian tiger (Panthera tigris altaica). Ma, Yuehui,Guan, Weijun,Lu, Taofeng,Ma, Qin,Zhu, Minghao,Chen, Hongyan,Sun, Yujiao,Ma, Jianzhang,Liu, Dan.

[7]Transcriptomic analysis reveals importance of ROS and phytohormones in response to short-term salinity stress in Populus tomentosa. Zheng, Lingyu,Ma, Jing,Zhao, Xiulian,Ji, Jing,Chang, Ermei,Deng, Nan,Shi, Shengqing,Jiang, Zeping,Meng, Yu,Cheng, Telong,Meng, Chen,Chen, Lanzhen,Chen, Lanzhen. 2015

[8]Transcriptomic analysis of the larva Taenia multiceps. Li, W. H.,Zhang, N. Z.,Yang, Y.,Li, L.,Yan, H. B.,Li, T. T.,Qu, Z. G.,Jia, W. Z.,Fu, B. Q.,Yue, L.,Jia, W. Z.,Fu, B. Q.. 2017

[9]Chemosensory Gene Families in Ectropis grisescens and Candidates for Detection of Type-II Sex Pheromones. Li, Zhao-Qun,Luo, Zong-Xiu,Cai, Xiao-Ming,Bian, Lei,Xin, Zhao-Jun,Liu, Yan,Chu, Bo,Chen, Zong-Mao. 2017

[10]Transcriptomic Analysis Reveals Candidate Genes for Female Sterility in Pomegranate Flowers. Chen, Lina,Zhang, Jie,Li, Haoxian,Niu, Juan,Xue, Hui,Liu, Beibei,Wang, Qi,Luo, Xiang,Zhang, Fuhong,Zhao, Diguang,Cao, Shangyin. 2017

[11]Protective efficacy of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhou, Dong-Hui,Zhu, Xing-Quan,Chen, Jia,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild. 2014

[12]Serum Metabolic Profiling of Oocyst-Induced Toxoplasma gondii Acute and Chronic Infections in Mice Using Mass-Spectrometry. Zhou, Chun-Xue,Chen, Xiao-Qing,Zhu, Xing-Quan,Zhou, Chun-Xue,He, Shen-Yi,Cong, Wei,Chen, Xiao-Qing,Elsheikha, Hany M.. 2018

[13]Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Petersen, Eskild,Huang, Si-Yang,Zhu, Xing-Quan. 2015

[14]Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis. Xue, Junxin,Xiao, Yan,Huang, Kehe,Xue, Junxin,Jiang, Wei,Chen, Yongjun,Liu, Yingchun,Zhang, Huajing,Wang, Quan,Qiao, Yuanbiao. 2016

[15]Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations. Ning, H. R.,Zhu, X. Q.,Ning, H. R.,Wang, J. L.,Qin, S. Y.,Huang, S. Y.,Lou, Z. L.,Hu, L. Y.,Zhu, X. Q.,Qin, S. Y.,Lou, Z. L.,Huang, S. Y.,Zhu, X. Q.. 2015

[16]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[17]Seroprevalence and genetic characterization of Toxoplasma gondii in masked palm civet (Paguma larvata) in Hainan province, tropical China. Hou, Guan-Yu,Zhou, Han-Lin,Rong, Guang,Zhao, Jun-Ming. 2016

[18]Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations. Ren, Di,Zhou, Yang,Lin, Ze-Ping,Lin, Rui-Qing,Wu, Song-Ming,Lin, Shao-Qi,Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Xu, Min-Jun,Zhou, Yang,Wu, Song-Ming,Yuan, Zi-Guo,Yang, Jian-Fa,Zou, Feng-Cai. 2012

[19]Seroprevalence, Isolation, Genotyping, and Pathogenicity of Toxoplasma gondii Strains from Sheep in China. Yang, YuRong,Feng, YongJie,Lu, YaoYao,Liang, HongDe,Zhang, LongXian,Yao, QiuXia,Zhu, XingQuan,Wang, YingHua. 2017

[20]Toxoplasma gondii Clp family protein: TgClpB1 plays a crucial role in thermotolerance. Cao, Shinuo,Du, Nali,Chen, Heming,Pang, Yu,Zhang, Zhaoxia,Zheng, Jun,Jia, Honglin. 2017

作者其他论文 更多>>

微信小程序