Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion

文献类型： 外文期刊

第一作者： Huai, Ya-Hong

作者： Huai, Ya-Hong;Xu, Shang-Zhong

作者机构：

关键词： Trf2 gene;cDNA cloning;Real-time fluorescence quantitative PCR;Bioinformation prediction

期刊名称：PROCEEDINGS OF THE 2016 INTERNATIONAL CONFERENCE ON BIOMEDICAL AND BIOLOGICAL ENGINEERING

ISSN：

年卷期： 2016 年

页码：

收录情况： SCI

摘要： In order to study Trf 2 protein function in the sperm production, the Trf2 gene was studied and the protein function was predicted in this paper. Based on blast searches with the mRNA sequence of human Trf 2 and the bovine-EST databases, we used fragment assembly and RT-PCR technology to amplify sequence. The results show that a 1701 bp cDNA fragment (GenBank accession No. EU140625) is first obtained. The sequence contains a 561 bp ORF encoding 186 amino acids. Homology analysis show that the amino acid sequences of bovine Trf 2 is highly homologous to other species. Real-time F Q-PCR demonstrates that the level of Trf 2 gene in liver and testis is highest in all organizations, relative to muscle. The protein may be associated with spermatogenesis. We acquire the Trf2 gene sequences and predict the function of Trf2 protein.

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