Purification and Characterization of a Thermostable beta-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases

文献类型： 外文期刊

第一作者： Cheng, Lifeng

作者： Cheng, Lifeng;Duan, Shengwen;Feng, Xiangyuan;Zheng, Ke;Yang, Qi;Liu, Zhengchu

作者机构：

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2016 年

页码：

收录情况： SCI

摘要： beta-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the beta-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified beta-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65 degrees C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70 degrees C and pH 4.5-7.0. The beta-mannanase activity was significantly enhanced in the presence of Mn2+, Cu2+, Zn2+, Ca2+, Mg2+, and Al3+ and strongly inhibited by Ba2+ and Pb2+ K-m and V-max values for locust bean gum were 7.14 mg/mL and 107.5 mu mol/min/mL versus 1.749 mg/mL and 33.45 mu mol/min/mL for Konjac glucomannan, respectively. Therefore, beta-mannanase purified by this work shows stability at high temperatures and in weakly acidic or neutral environments. Based on such data, the beta-mannanase will have potential applications as a dietary supplement in treatment of inflammatory processes.

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