Screening and chromosome localization of two cotton BAC clones

文献类型： 外文期刊

第一作者： Xinglei Cui

作者： Xinglei Cui;Fang Liu;Yuling Liu;Zhongli Zhou;Chunying Wang;Yanyan Zhao;Fei Meng;Xingxing Wang;Xiaoyan Cai;Yuhong Wang;Renhai Peng;Kunbo Wang

作者机构：

关键词： Cotton;BAC;FISH;cytological marker;microcolinearity

期刊名称：COMPARATIVE CYTOGENETICS （ 影响因子：1.8； 五年影响因子：1.438 ）

ISSN： 1993-0771

年卷期： 2016 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Two bacterial artificial chromosome (BAC) clones (350B21 and 299N22) of Pima 90-53 cotton [Gossypium barbadense Linnaeus, 1753 (2n=4x=52)] were screened from a BAC library using SSR markers. Strong hybridization signals were detected at terminal regions of all A genome (sub-genome) chromosomes, but were almost absent in D genome (sub-genome) chromosomes with BAC clone 350B21 as the probe. The results indicate that specific sequences, which only exist at the terminal parts of A genome (sub-genome) chromosomes with a huge repeat number, may be contained in BAC clone 350B21. When utilizing FISH with the BAC clone 299N22 as probe, a pair of obvious signals was detected on chromosome 13 of D genome (sub-genome), while strong dispersed signals were detected on all A genome (sub-genome) chromosomes. The results showed that peculiar repetitive sequence, which was distributed throughout all A genome (sub-genome) chromosomes, may exist in BAC clone 299N22. The absence of the repetitive sequences, which exist in the two BAC clones, in D genome may account for the genome-size variation between A and D genomes. In addition, the microcolinearity analysis of the clone 299N22 and its homologous region on G. raimondii Ulbrich, 1932 chromosome 13 (D(5)13) indicated that the clone 299N22 might come from A sub-genome of sea island cotton (G. barbadense), and a huge number of small deletions, illegitimate recombination, translocation and rearrangements may have occurred during the genus evolution. The two BAC clones studied here can be used as cytological markers but will be also be helpful to research in cotton genome evolution and comparative genomics.

分类号：

  • 相关文献

[1]Cytogenetic maps of homoeologous chromosomes A(h)01 and D(h)01 and their integration with the genome assembly in Gossypium hirsutum. Yuling Liu,Liu, Fang,Wang, Kunbo,Zhen Liu,Renhai Peng,Yuhong Wang,Zhongli Zhou,Xiaoyan Cai,Xingxing Wang,Zhenmei Zhang,Kunbo Wang,Fang Liu. 2017

[2]A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons. Yuling Liu,Renhai Peng,Fang Liu,Xingxing Wang,Xinglei Cui,Zhongli Zhou,Chunying Wang,Xiaoyan Cai,Yuhong Wang,Zhongxu Lin,Kunbo Wang. 2016

[3]Microdissection and Microcloning of Chromosome 5 in Gossypium arboreum. Peng Renhai,Liu Fang,Hu Xiao,Wang Chunying,Li Shaohui,Zhang Xiangdi,Wang Yuhong,Wang Kunbo.

[4]Cloning of equine herpesvirus type 1 438/77 strain genome as a bacterial artificial chromosome. Sun, Xuegiang,Yao, Huochun,Lu, Chengping,Zhang, Cun.

[5]New cis-regulatory elements in the Rht-D1b locus region of wheat. Duan, Jialei,Wu, Jiajie,Liu, Yue,Xiao, Jianhui,Zhao, Guangyao,Jia, Jizeng,Kong, Xiuying,Gu, Yongqiang,Duan, Jialei. 2012

[6]Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Shao Changwei,Shao Changwei,Liu Shanshan,Liu Changlin,Chen Songlin,Liu Geng. 2013

[7]Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD. Su, S.,Cui, N.,Sun, P.,Cui, Z.,Li, J.,Li, H.,Li, Y..

[8]Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization. Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,You, Weiwei,Ke, Caihuan,Zhang, Xiaojun,Jiang, Likun,Chen, Baohua,Mahboob, Shahid,Al-Ghanim, Khalid A.,Mahboob, Shahid.

[9]Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus. Yu, Zu-Hua,Teng, Man,Yu, Le-Le,Hu, Bo,Zhang, Gai-Ping,Luo, Jun,Yu, Zu-Hua,Hu, Bo,Liu, Ju-Xiong,Zhang, Gai-Ping,Sun, Ai-Jun,Cui, Zhi-Zhong,Qu, Liang-Hu,Yu, Zu-Hua,Ding, Ke,Cheng, Xiang-Chao,Luo, Jun.

[10]Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library. Xia, Zhengjun,Wu, Hongyan,Xia, Zhengjun,Watanabe, Satoshi,Harada, Kyuya.

[11]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[12]1-Deoxynojirimycin from Bacillus subtilis improves antioxidant and antibacterial activities of juvenile Yoshitomi tilapia. Tang, Lining,Huang, Kai,Sun, Lei,Huang, Qing,Bi, Yanjun,Xie, Jun,Yu, Dan. 2017

[13]Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background. Yang, Zu-Jun,Li, Guang-Rong,Zeng, Zi-Xian,Zhang, Yong,Zhou, Jian-Ping,Liu, Zhao-Hui,Ren, Zheng-Long. 2008

[14]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[15]Karyotyping in Melon (Cucumis melo L.) by Cross-Species Fosmid Fluorescence in situ Hybridization. Liu, C.,Liu, J.,Li, H.,Han, Y.,Jin, W.,Zhang, Z.,Huang, S.,Liu, J.. 2010

[16]Metals in the Tissues of Two Fish Species from the Rare and Endemic Fish Nature Reserve in the Upper Reaches of the Yangtze River, China. Cai, Shenwen,Ni, Zhaohui,Li, Yunfeng,Shen, Ziwei,Zhang, Yan,Zhou, Yuntao,Cai, Shenwen,Xiong, Zhiting. 2012

[17]Use of chitin and krill in aquaculture - the effect on gut microbiota and the immune system: a review. Ringo, E.,Zhou, Z.,Olsen, R. E.,Song, S. K.. 2012

[18]Evaluating the toxicity of environmental concentrations of waterborne chromium (VI) to a model teleost, oncorhynchus mykiss: a comparative study of in vivo and in vitro. Li, Zhi-Hua,Li, Ping,Randak, Tomas,Li, Zhi-Hua,Li, Ping. 2011

[19]Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion. Hongtuo, F,Chingjiang, W. 2001

[20]Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH. Zhao, Yan,Wang, Junjie,Zhang, Zhong,Zhao, Yan,Wang, Junjie,Zhang, Zhong,Zhao, Yan,Wang, Junjie,Zhang, Zhong,Xie, Jihong,Dou, Quanwen. 2017

作者其他论文 更多>>

微信小程序