Isolation and characterization of a herpesvirus from feral pigeons in China

文献类型: 外文期刊

第一作者: Zhao, Panpan

作者: Zhao, Panpan;Ma, Jian;Guo, Ying;Tian, Li;Guo, Guangyang;Zhang, Kexin;Xing, Mingwei;Ma, Jian

作者机构:

关键词: Columba livia f. dom;Herpesvirus;Phylogenetic analysis;Polymerase chain reaction;Transmission electron microscopy

期刊名称:VETERINARY JOURNAL ( 影响因子:2.688; 五年影响因子:2.857 )

ISSN: 1090-0233

年卷期: 2015 年 206 卷 3 期

页码:

收录情况: SCI

摘要: A herpesvirus was isolated during a diagnostic investigation of severe cases of conjunctivitis in feral pigeons (Columba livia f. domestica). Isolates of the virus were recovered from throat swabs of the pigeons followed by inoculation of the swab samples in chicken embryo fibroblasts. Pigeons inoculated with the isolated virus had similar clinical signs to those observed in naturally infected birds. Transmission electron microscopy revealed viral structures with typical herpesvirus morphology. Polymerase chain reaction amplification, using herpesvirus-identifying primers resulted in an amplicon of the expected size for herpesvirus. Sequencing of these amplicons and database comparisons identified the herpesvirus UL30 homologue. Phylogenetic reconstructions suggested that the isolated herpesvirus belongs to the Mardivirus genus of Alphaherpesvirinae. Using the current herpesvirus nomenclature conventions, the authors propose that the herpesvirus be named Columbid herpesvirus-1 Heilongjiang. (C) 2015 Elsevier Ltd. All rights reserved.

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