Three phase partitioning for simultaneous purification of aloe polysaccharide and protein using a single-step extraction

文献类型： 外文期刊

第一作者： Tan, Zhi-Jian

作者： Tan, Zhi-Jian;Wang, Chao-Yun;Yi, Yong-Jian;Wang, Hong-Ying;Zhou, Wan-Lai;Tan, Shi-Yong;Tan, Zhi-Jian;Li, Fen-Fang

作者机构：

关键词： Three phase partitioning;Aloe polysaccharide;Aloe protein;Purification

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2015 年 50 卷 3 期

页码：

收录情况： SCI

摘要： In this paper, an inexpensive, rapid and efficient three phase partitioning (TPP) technique was used to simultaneously purify aloe polysaccharide (APS) and protein using a single-step extraction. This TPP system is established by adding ammonium sulfate and t-butanol to the crude slurry of aloe powder. APS was extracted in the lower phase, and the protein was extracted in the middle phase. The extraction conditions optimized were mass concentration of ammonium sulfate and t-butanol, temperature and pH. APS was further purified using a dialysis membrane to remove the salt, and its purity was determined. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze aloe protein. TPP is an attractive and potential technique for the purification of APS and protein from Aloe vera L. (C) 2015 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. Ma, Duanzheng,Wang, Hexiang,Sekete, Malota,Ma, Duanzheng,Wang, Hexiang,Wang, Bo,Ng, Tzibun. 2012

[2]Purification and characterization of a cis-epoxysuccinic acid hydrolase from Bordetella sp strain 1-3. Li, Xia,Lu, Hongbo,Ma, Xiaohang,Kai, Lei,Guo, Kangping,Zhao, Yuhua,Li, Xia,Xu, Tongcheng. 2010

[3]An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea. Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Wang, Bo,Gong, Zhiyuan,Ng, Tzi Bun. 2014

[4]Purification, characterization, and complement fixation activity of acidic polysaccharides from Tuber sinoaestivum. Zhang, Bo,Zhou, Jie,He, Xiaolan,Ye, Lei,Zou, Jie,Wu, Chenguang,Peng, Weihong,Zhang, Bo,Zou, Jie,Wu, Chenguang,Zhang, Xiaoping,Li, Juan.

[5]Molecular cloning of dihydroflavonol 4-reductase gene from grape berry and preparation of an anti-DFR polyclonal antibody. Zhang, Ping,Wen, Peng-Fei,Wan, Si-Bao,Wang, Wei,Pan, Qiu-Hong,Zhan, Ji-Chen,Huang, Wei-Dong,Zhang, Ping,Wen, Peng-Fei. 2008

[6]Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes). Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi. 2013

[7]Preparation, purification and regeneration optimizing research of protoplasts from Rhizoctonia solani. Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping,Li, HaoJie,Feng, Haitao,Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping. 2012

[8]Ultrasonic-assisted extraction of sinomenine from Sinomenium acutum using magnetic ionic liquids coupled with further purification by reversed micellar extraction. Wang, Chaoyun,Yi, Yongjian,Zhou, Wanlai,Wang, Hongying,Tan, Zhijian,Wang, Chaoyun,Yi, Yongjian,Zhou, Wanlai,Wang, Hongying,Tan, Zhijian,Li, Qiao,Wu, Shuanggen,Li, Fenfang. 2017

[9]Purification and characterisation of a phosphatidylcholine-binding protein from duck Biceps femoris muscle. Wang, D. Y.,Zhang, M. H.,Liu, F.,Zhu, Y. Z.,Xu, W. M.. 2014

[10]A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings. Liu Xin-gang,Dong Feng-shou,Xu Jun,Guo Li-qun,Kong Zhi-qiang,Tian Ying-ying,Zheng Yong-quan,Wu Yan-bin. 2013

[11]Purification and properties of lysozyme from a marine strain. Zhang, Xiu,Ren, Xian,Zhang, Xiu,Wang, Yue-Jun,Sun, Mi,Zou, Yan-Li,Wang, Qing-Yin,Wang, Wei. 2008

[12]Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916. Chen, Zhiyi,Ng, T. B.,Zhang, Jie,Zhou, Mingguo,Song, Fuping,Lu, Fan,Liu, Youzhou. 2007

[13]Extraction, Purification and partial characterizations of Polysaccharides from Gracilaria lemaneiformis. Yang, Xianqing,Liu, Mingqiu,Qi, Bo,Li, Laihao,Deng, Jianchao,Hu, Xiao,Wu, Yanyan,Hao, Shuxian. 2014

[14]Protein Cyclization Enhanced Thermostability and Exopeptidase-Resistance of Green Fluorescent Protein. Zhao Zhonglin,Ma, Xin,Li, Liang,Zhang, Wei,Ping, Shuzhen,Lin, Min,Xu, Ming-Qun. 2010

[15]Comparison of cerato-platanin family protein BcSpl1 produced in Pichia pastoris and Escherichia coli. Liang, Yingbo,Qiu, Dewen,Yuan, Jingjing,Yang, Xiufen. 2017

[16]Biochemical characteristics of a novel protease from the basidiomycete Amanita virgineoides. Yuan, Xianghe,Wang, Hexiang,Yuan, Xianghe,Wang, Hexiang,Zhu, Mengjuan,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2017

[17]Purification and characterization of a psychrophilic catalase from Antarctic Bacillus. Wang, Wei,Sun, Mi,Zhang, Bin,Liu, Wanshun. 2008

[18]Isolation, Purification, Antioxidant and Immunological Activities in Vitro of Polysaccharide from Acanthopanacis Senticosi. Chen Ruizhan,Dong Hang,Meng Fanlei,Liu Zhiqiang. 2011

[19]Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia. Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Zhu, Peilei. 2018

[20]Purification and characteristics of serine protease from the head of pacific white shrimp. Wu, Yanyan,Li, Laihao,Yang, Xianqing,Diao, Shiqiang,Wang, Ping. 2012

作者其他论文 更多>>

微信小程序