Comparative analysis of polygalacturonase in the fruit of strawberry cultivars

文献类型： 外文期刊

第一作者： Zhou, H. C.

作者： Zhou, H. C.;Li, G.;Zhao, X.;Li, L. J.

作者机构：

关键词： Strawberry;Polygalacturonase;Fruit texture;Fruit ripening and softening;Gene expression analysis

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： The role of polygalacturonase (PG) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. Changes in PG activity and gene expression during development, ripening and softening were measured. The PG genes from each cultivar were cloned and analyzed, and were classified with other PG genes using phylogenetic analysis. In Toyonoka fruit, PG activity increased gradually, reaching a peak during the pink stage, and remained at this level during post-harvest softening. Changes in PG gene expression were consistent with PG activity in these softer fruits. In the firmer Sweet Charlie fruits, PG activity was detected during the initial development stage, reaching a peak at the white stage, thereafter decreasing gradually with ripening and remaining at this lower level throughout softening. Changes in PG gene expression and PG activity were not consistent in these fruit. For both Toyonoka and Sweet Charlie PG genes (FaTPG and FaSCPG, respectively), the open reading frame was 1218 bp, encoding 405 amino acids. Five different nucleotide sites were observed between the two sequences, leading to two amino acid sequence mutations. FaTPG, FaSCPG, and PG genes from the Fragaria vesca genome were classified into three clades using phylogenetic analysis. The clade containing PG genes involved in fruit softening had functional similarity but there were no functional differences between FaTPG and FaSCPG. Differences in PG activity, gene sequence, and gene expression may have led to different roles of PG during ripening and softening in strawberries with different textures.

分类号：

  • 相关文献

[1]Comparative analysis of pectate lyase in relation to softening in strawberry fruits. Zhou, Houcheng,Li, Gang,Zhao, Xia. 2016

[2]Combined herbicide and saline stress differentially modulates hormonal regulation and antioxidant defense system in Oryza sativa cultivars. Islam, Faisal,Ali, Basharat,Wang, Jian,Farooq, Muhammad A.,Gill, Rafaqat A.,Zhou, Weijun,Islam, Faisal,Ali, Basharat,Wang, Jian,Farooq, Muhammad A.,Gill, Rafaqat A.,Zhou, Weijun,Ali, Shafaqat,Wang, Danying.

[3]Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz). Yao, Yuan,Geng, Meng-Ting,Liu, Jiao,Li, Rui-Mei,Guo, Jian-Chun,Wu, Xiao-Hui,Hu, Xin-Wen. 2014

[4]Genome-Wide Identification and Analysis of the Type-B Authentic Response Regulator Gene Family in Peach (Prunus persica). Zeng, Jingjue,Zhu, Xudong,Haider, Muhammad S.,Zhang, Cheng,Wang, Chen,Wang, Xicheng.

[5]Identification of volatile and softening-related genes using digital gene expression profiles in melting peach. Li, Xiong-wei,Jiang, Jun,Zhang, Li-ping,Yu, Yi,Chai, Ming-liang,Jia, Hui-juan,Gao, Zhong-shan,Li, Xiong-wei,Ye, Zheng-wen,Wang, Xiu-min,Zhou, Jing-yi,Zhang, Hui-qin,Arus, Pere. 2015

[6]Purification and characterization of polygalacturonase-inhibiting protein from Asian pear varieties. Tian, J. B.,Pastore, M.,Greve, L. C.,Vicente, A.,Labavitch, J. M.,Gregori, R.. 2008

[7]Functional analysis of two polygalacturonase genes in Apolygus lucorum associated with eliciting plant injury using RNA interference. Liu, Bing,Lu, Yanhui,Liang, Gemei.

[8]Two acidic, thermophilic GH28 polygalacturonases from Talaromyces leycettanus JCM 12802 with application potentials for grape juice clarification. Li, Yeqing,Xu, Bo,Li, Yeqing,Wang, Yuan,Tu, Tao,Zhang, Duoduo,Ma, Rui,You, Shuai,Wang, Xiaoyu,Yao, Bin,Luo, Huiying. 2017

[9]Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1. Pan, Xia,Li, Ke,Ma, Rui,Shi, Pengjun,Huang, Huoqing,Yang, Peilong,Meng, Kun,Yao, Bin,Ma, Rui,Yang, Peilong.

[10]Hot Water Treatment Maintains Normal Ripening and Cell Wall Metabolism in Mango (Mangifera indica L.) Fruit. Zhang, Zhengke,Gao, Zhaoyin,Li, Min,Hu, Meijiao,Gao, Hui,Yang, Dongping,Yang, Bo. 2012

[11]A novel bifunctional pectinase from Penicillium oxalicum SX6 with separate pectin methylesterase and polygalacturonase catalytic domains. Tu, Tao,Bai, Yingguo,Luo, Huiying,Ma, Rui,Wang, Yaru,Shi, Pengjun,Yang, Peilong,Meng, Kun,Yao, Bin.

[12]DRAMATICALLY REDUCED VIRULENCE OF MUTANTS OF PSEUDOMONAS-SOLANACEARUM DEFECTIVE IN EXPORT OF EXTRACELLULAR PROTEINS ACROSS THE OUTER-MEMBRANE. KANG, YW,HUANG, JZ,MAO, GZ,HE, LY,SCHELL, MA. 1994

[13]Circular Multi-Storied Culture Mode among Strawberry, Mushroom and Leaf Vegetables in Greenhouse. Zhong, Chuanfei,Zhang, Yuntao,Wang, Guixia,Dong, Jing,Chang, Linlin,Wang, Lina. 2011

[14]Genetically Transformed Strawberry (Fragaria x ananassa Duch.) with Cold-Inducible Transcription Factor CBF1. Jin, W. M.,Dong, J.,Liu, Y.,Zhang, Y. P.,Pan, Q. H.. 2009

[15]Study of Volatile Compound Contents in a Progeny Issued from Cross between 'Camarosa' and 'Benihoppe'. Wang, Guixia,Zhang, Yuntao,Dong, Jing,Zhong, Chuanfei,Chang, Linlin,Wang, Lina. 2011

[16]Inheritance of Fruit Characters in the Strawberry (Fragaria xananassa Duch.). Wang, Z. W.,Zhao, M. Z.,Yuan, J.,Qian, Y. M.,Wang, J.. 2014

[17]RdreB1BI enhances drought tolerance by activating AQP-related genes in transgenic strawberry. Gu, Xianbin,Gao, Zhihong,Yan, Yichao,Qiao, Yushan,Gu, Xianbin,Chen, Yahua,Wang, Xiuyun,Gu, Xianbin. 2017

[18]Study on the Production and Consumption of Strawberry in Beijing. Wang, L. N.,Zhang, Y. T.,Zhong, C. F.,Wang, G. X.,Dong, J.,Chang, L. L.. 2014

[19]Genome-wide identification and analysis of FK506-binding protein family gene family in strawberry (Fragaria x ananassa). Leng, Xiangpeng,Liu, Dan,Sun, Xin,Li, Yu,Mu, Qian,Zhu, Xudong,Li, Pengyu,Fang, Jinggui,Zhao, Mizhen. 2014

[20]Optimization of postharvest ultrasonic treatment of strawberry fruit. Cao, Shifeng,Hu, Zhichao,Pang, Bin. 2010

作者其他论文 更多>>

微信小程序