A possible way that phi C31 integrase regulates the recombination direction

文献类型： 外文期刊

第一作者： Liu, T. A.

作者： Liu, T. A.;Fang, Y. X.;Yin, H.;Jing, Z. Z.;Yin, H.;Jing, Z. Z.

作者机构：

关键词： phi C31 integrases;Hyperactive mutants;Synapsis;Directional control

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： phi C31 integrase encoded by Streptomyces phage can mediate site-specific recombination between phage and host genomes. The recombination direction is generally considered to be accurately regulated, but the regulatory mechanisms involved are still unclear. Recently, some hyperactive mutants of phi C31 integrase that can bypass the regulatory steps have been isolated and extensively studied. A putative coiled-coil region is found to play a critical role in controlling recombination direction. Further analysis led us to the speculation that at least two regions in the N-terminal domain of phi C31 integrase are involved in the tetrameric interfaces and that the putative coiled coil interacts with one of the regions to regulate the recombination direction.

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