Merozoite proteins from Babesia sp BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA

文献类型： 外文期刊

第一作者： Guan, G. Q.

作者： Guan, G. Q.;Chauvin, A.;Moreau, E.;Guan, G. Q.;Luo, J. X.;Yin, H.;Rogniaux, H.

作者机构：

关键词： Babesia sp BQ1 (Lintan);antigen;ELISA;merozoite;cross-reaction;antibody kinetics;prevalence

期刊名称：PARASITOLOGY （ 影响因子：3.234； 五年影响因子：3.118 ）

ISSN： 0031-1820

年卷期： 2010 年 137 卷 6 期

页码：

收录情况： SCI

摘要： Babesia sp BQ1 (Lintan) is Babesia isolated from sheep infested with Haemaphysalis qinghatensis in China, and is closely related to B motasi, based on the I SS rRNA gene sequence In the present study, an ELISA was des eloped with merozoite antigens of Babesia sp BQ1 (Liman) (BQMA) purified horn in vino culture When the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95 5% Except for Babasia sp Tianzhu, there was no cross-reaction between BQMA and positive sera from Babesia sp BQI (Ningxian)-, Babesia sp Babesia sp Xinjiang-, Theileria luwenshum-, T utlenbergi-. or Anaplasma ovis-infected sheep, which are the dominant haemoparasites of small ruminants in China Specific antibodies against Babesia sp BQ1 I (Liman) were produced 1 or 2 weeks post-Infection and a high level of antibodies persisted for more than 8 months in experimentally infected sheep This ELISA was tested on 974 sera collected from field-grazing sheep in 3 counties of Gansu province, northwestern China to evaluate the seroprevalence of Babesia sp 1301 (Lintan) Infection and the average positive rate was 66 84% The feasibility of increasing the specificity of this BQMA-based ELISA, by using some BQMA antigens for serodiagnosis is discussed

分类号：

  • 相关文献

[1]Seroprevalence of bovine theileriosis in northern China. Li, Yaqiong,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Guo, Pengfei,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Li, Youquan,Yin, Hong. 2016

[2]Evaluating an indirect rMPSP enzyme-linked immunosorbent assay for the detection of bovine Theileria infection in China. Zhao, Shuaiyang,Liu, Junlong,Zhao, Hongxi,Li, Youquan,Xie, Junren,Liu, Aihong,Hassan, Muhammad-Adeel,Yin, Hong,Guan, Guiquan,Luo, Jianxun,Zhao, Hongxi,Yin, Hong.

[3]A member of the HSP90 family from ovine Babesia in China: molecular characterization, phylogenetic analysis and antigenicity. Guan, Guiquan,Liu, Junlong,Liu, Aihong,Li, Youquan,Niu, Qingli,Gao, Jinliang,Luo, Jianxun,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Yin, Hong. 2015

[4]Expression analysis and biological characterization of Babesia sp BQ1 (Lintan) (Babesia motasi-like) rhoptry-associated protein 1 and its potential use in serodiagnosis via ELISA. Niu, Qingli,Liu, Zhijie,Yang, Jifei,Yu, Peifa,Pan, Yuping,Zhai, Bintao,Luo, Jianxun,Guan, Guiquan,Yin, Hong,Yin, Hong,Moreau, Emmanuelle. 2016

[5]Small-scale Expressed Sequence Tag Analysis Of Theileria Uilenbergi: Identification Of A Gene Family Encoding Potential Antigenic Proteins. Liu, Zhijie,Ahmed, Jabbar S.,Seitzer, Ulrike,Liu, Zhijie,Dang, Zhisheng,Luo, Jianxian,Yin, Hong.

[6]Identification and characterization of Tu88, an antigenic gene from Theileria uilenbergi. Lu, Yizhu,Wang, Yuefeng,Li, Youquan,Gou, Huitian,Luo, Jianxun,Yin, Hong,Liu, Zhijie,Yin, Hong.

[7]Expression of an immunologically (MSP-1(42)) reactive merozoite surface protein in E-coli. Leung, WH,Meng, ZQ,Hui, G,Ho, WK.

[8]Dual-competitive lateral flow aptasensor for detection of aflatoxin B-1 in food and feedstuffs. Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Huang, Yafei,Ren, Shuyue,Gao, Zhixian. 2018

[9]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006

[10]Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens. Zhang, De-Lin.

[11]Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[12]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[13]Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China. Song, Yansu,Hong, Ni,Wang, Guoping,Song, Yansu,Hong, Ni,Wang, Liping,Xu, Wenxing,Ding, Fang,Wang, Guoping,Hu, Hongju,Tian, Rui.

[14]A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Guan, Guiquan,Hassan, Muhammad Adeel,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2017

[15]Porcine circovirus type 2 in China: an update on and insights to its prevalence and control. Zhai, Shao-Lun,Sun, Bei-Bei,Deng, Su-Fang,Hu, Jun,Luo, Man-Lin,Zhai, Shao-Lun,Xu, Zhi-Hong,Lv, Dian-Hong,Wen, Xiao-Hui,Yuan, Jie,Wei, Wen-Kang,Chen, Sheng-Nan,Tang, Man-Hua,Wang, Feng-Guo,Li, Xiao-Jing. 2014

[16]Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China. Yan, Chao,Yue, Cai-Ling,Wang, Dong-Hui,Zhou, Dong-Hui,Yan, Chao,Fu, Lin-Lin,Yue, Cai-Ling,Tang, Ren-Xian,Liu, Yi-Sheng,Zhang, Peng,Wang, Dong-Hui,Zheng, Kui-Yang,Lv, Liang,Shi, Na,Zeng, Ping,Zhu, Xing-Quan,Zhu, Xing-Quan. 2012

[17]Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis. Wang, Ze-Dong,Wei, Feng,Wang, Ze-Dong,Zhu, Xing-Quan,Wang, Ze-Dong,Liu, Huan-Huan,Ma, Hong-Yu,Li, Zhong-Yu,Liu, Quan,Ma, Zhan-Xi,Yang, Zhi-Bin,Xu, Bin,Liu, Quan. 2017

[18]Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Wen, Libin,He, Kongwang,Yu, Zhengyu,Mao, Aihua,Zhong, Shulin,Ni, Yanxiu,Zhang, Xuehan,Li, Bin,Wang, Xiaomin,Zhou, Junming,Guo, Rongli,Lv, Lixin,Jiang, Jieyuan,Yang, Hanchun. 2011

[19]Prevalence of Hydatid Cysts in Livestock Animals in Xinjiang, China. Meng Qingling,Qiao Jun,Liu Tianli,Song Xuemei,Chen Chuangfu,Zhu Xinquan,Wang Guanglei,Zhang Jinsheng,Wang Huisheng,Cai Kuojun.

[20]Prevalence, intensity and associated factor analysis of Tropilaelaps mercedesae infesting Apis mellifera in China. Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang,Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang.

作者其他论文 更多>>

微信小程序