Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Rong, Guangyu;Guo, Chen;Luo, Xiaomei;Kang, Hong;Sun, Yanwei;Wang, Xiaohua;Wang, Xin;Sun, Qingshen;Rong, Guangyu;Sun, Yanwei;Cui, Shangjin;Dai, Chunxiao;Jin, Zheng

作者机构：

关键词： LDH@SiO2 nanoparticles;Newcastle disease;mucosal immunity;Cytotoxicity;IgA

期刊名称：INTERNATIONAL JOURNAL OF NANOMEDICINE （ 影响因子：6.4； 五年影响因子：6.761 ）

ISSN： 1178-2013

年卷期： 2015 年 10 卷

页码：

收录情况： SCI

摘要： Layered double hydroxide (LDH)@SiO2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO2 nanoparticles (pFDNA-LDH@SiO2-NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO2-NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO2-NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%+/- 0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO(2-)NPs within 288 hours. The LDH@SiO2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO2-NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F48E9 with highly virulent (mean death time of chicken embryos. 60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

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