Ultra-performance LC Separation and Quadrupole Time-of-flight MS Identification of Major Alkaloids in Plumula Nelumbinis

文献类型： 外文期刊

第一作者： Lin, Zongtao

作者： Lin, Zongtao;Yang, Ruinan;Li, Wei;Guan, Zheng;Chen, Ailiang

作者机构：

关键词： QTOF;MS;ultra-performance liquid chromatography;alkaloids;Plumula Nelumbinis

期刊名称：PHYTOCHEMICAL ANALYSIS （ 影响因子：3.373； 五年影响因子：2.959 ）

ISSN： 0958-0344

年卷期： 2014 年 25 卷 6 期

页码：

收录情况： SCI

摘要： IntroductionAs an essential medicine and tea source in many countries, Plumula Nelumbinis potentially exerts its major biological activities through its alkaloids. However, the activities of Plumula Nelumbinis are not fully understood due to the lack of studies on its chemical components. ObjectiveTo establish an ultra-performance liquid chromatography combined with diode-array detector (UPLC/DAD) method, coupled to an electrospray ionisation with quadrupole time-of-flight mass spectrometry (ESI/QTOF/MS) method, for the separation and identification of Plumula Nelumbinis alkaloids. MethodsThe eluant from an UPLC separation of an ethanol extract of Plumula Nelumbinis was directly infused into an ESI/QTOF/MS system. Both positive and negative ion modes of ESI with low and high collision energy (CE) were used to obtain sufficient MS information. ResultsTwenty-one alkaloids were tentatively identified based on their chromatographic characteristics, UV spectra, exact mass, MS fragments and literature reports. They consist of six bis-1-benzyltetrahydroisoquinoline, eleven benzyltetrahydroisoquinoline (including two glycoalkaloids and two quaternary ammoniums), two aporphine, one proaporphine and one indole alkaloids. Eleven were identified in Plumula Nelumbinis for the first time and seven were first reported in Nelumbo nucifera Gaertn. Five compounds, namely norcoclaurine-4-O-glucoside, norcoclaurine-6-O-glucoside, isolotusine, 6-demethyl-4-demethylN-methylcoclaurine and N-norisoliensinine, were characterised and proposed as new compounds. ConclusionThe established UPLC/DAD-ESI/QTOF/MS method is efficient for systematic identification of the alkaloids in Plumula Nelumbinis extract. Copyright (c) 2014 John Wiley & Sons, Ltd. The alkaloid components from the embryo of the seed of Nelumbo nucifera Gaertn. (Plumula Nelumbinis) were analyzed using UPLC/DAD-ESI/QTOF/MS techniques. Twenty-one alkaloids, including 6 bis-1-benzyltetrahydroisoquinolines, 11 benzyltetrahydroisoquinolines, 2 aporphines, 1 proaporphine and 1 indole, were tentatively identified in the sample. Of these, eleven were first identified in Plumula Nelumbinis; seven were first identified in the plant Nelumbo nucifera Gaertn.; five alkaloids were tentatively identified and proposed as new natural products.

分类号：

  • 相关文献

[1]Expression Analysis of Anthocyanin Biosynthetic Genes in Different Colored Sweet Cherries (Prunus avium L.) During Fruit Development. Liu, Yun,Shen, Xinjie,Zhao, Kai,Guo, Xinwei,Li, Tianhong,Ben, Yue,Zhang, Xiaoming. 2013

[2]UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts. Xu, Rui,Mao, Fuying,Zhao, Yunsheng,Wang, Wenping,Fan, Lingling,Gao, Xiaojuan,Zhao, Jianjun,Mao, Fuying,Zhao, Yunsheng,Wang, Wenping,Gao, Xiaojuan,Zhao, Jianjun,Zhao, Yunsheng,Tian, Hongling. 2017

[3]Influence of pH, concentration and light on stability of allicin in garlic (Allium sativum L.) aqueous extract as measured by UPLC. Wang, Haiping,Li, Xixiang,Liu, Xinyan,Shen, Di,Qiu, Yang,Zhang, Xiaohui,Song, Jiangping.

[4]Determination of neonicotinoid insecticides residues in eels using subcritical water extraction and ultra-performance liquid chromatography-tandem mass spectrometry. Xiao, Zhiming,Yang, Yunxia,Li, Yang,Fan, Xia,Ding, Shuangying.

[5]Analysis of free amino acids in Amur sturgeon by ultra-performance liquid chromatography using pre-column derivatization with 6-aminoquinolyl-carbamyl. Sun, Yanchun,Xu, Xianzhu,Sun, Yanchun,Mou, Zhenbo,Wu, Song,Wang, Jing,Tan, Zhijun.

[6]Simultaneous determination of pyrethrins residues in teas by ultra-performance liquid chromatography/tandem mass spectrometry. Liu, Xingang,Dong, Fengshou,Xu, Jun,Zhang, Changpeng,Li, Yuanbo,Zheng, Yongquan,Song, Wencheng.

[7]HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia. Yao, LH,Jiang, YM,Datta, N,Singanusong, R,Liu, X,Duan, J,Raymont, K,Lisle, A,Xu, Y. 2004

[8]Bioassay-guided isolation and identification of active compounds from Macleaya microcarpa (Maxim) Fedde against fish pathogenic bacteria. Kang, Yu-Jun,Yi, Yang-lei,Zhang, Chao,Wang, Gao-Xue,Wu, Shu-Qin,Shi, Cun-Bin. 2013

[9]Phytochemistry and Pharmacological Activities of the Genus Gentiana (Gentianaceae). Pan, Yu,Zhao, Yan-Li,Zhang, Ji,Li, Wan-Yi,Wang, Yuan-Zhong,Pan, Yu.

[10]Novel Cyclic Hexapeptides from Marine-Derived Fungus, Aspergillus sclerotiorum PT06-1. Zheng, Jinkai,Wang, Yi,Liu, Peipei,Wang, Xin,Peng, Xiaoping,Zhu, Weiming,Zhu, Huajie,Hong, Kui.

[11]Detoxification of gramine by the cereal aphid Sitobion avenae. Cai, Qing-Nian,Han, Ying,Hu, Yuan,Zhao, Xin,Cao, Ya-Zhong.

[12]Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry. Zhang, Li-li,Shu, Shu-lan,Qian, Da-wei,Duan, Jin-ao,Zhang, Li-li,Shu, Shu-lan,Qian, Da-wei,Duan, Jin-ao,Bai, Yong-liang,Zhen Ou-yang,Liu, Li.

[13]Antifungal Efficacy of Phellodendron amurense Ethanol Extract against Trichophyton mentagrophytes in Rabbits. Xiao, C. W.,Ji, Q. A.,Wei, Q.,Liu, Y.,Bao, G. L.,Rajput, Z. I.. 2014

[14]Tetracyclic indole alkaloids with antinematode activity from Uncaria rhynchophylla. Kong, Fandong,Ma, Qingyun,Huang, Shengzhuo,Zhou, Liman,Dai, Haofu,Zhao, Youxing,Yang, Shuang,Fu, Linran,Yu, Zhifang. 2017

[15]DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION. Chen, Sha,Liu, Yanling,Li, Shaohua,Chen, Sha,Zhang, Haohao,Fang, Jinbao.

[16]Two Stemona alkaloids from Stemona sessilifolia (Miq.) Miq.. Dong, Jian-Li,Yang, Zhong-Duo,Xue, Hong-Yan,Shu, Zong-Mei,Zhou, Shuang-Yan,Yao, Xiao-Jun,Xue, Hong-Yan.

[17]Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD-ESI/MS. Liu, Guozhu,Chen, Bo,Yao, Shouzhuo,He, Dongxiu,Shan, Yang,Wu, Yuehui.

[18]Microwave-assisted extraction of three bioactive alkaloids from Peganum harmala L. and their acaricidal activity against Psoroptes cuniculi in vitro. Shang, Xiaofei,Guo, Xiao,Li, Bing,Pan, Hu,Zhang, Jiyu,Miao, Xiaolou,Zhang, Yu.

[19]Anti-endotoxin and anti-inflammatory effects of Chinese herbal medicinal alkaloid ingredients in vivo. Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang,Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang.

[20]Trigonelline, a naturally occurring constituent of green coffee beans behind the mutagenic activity of roasted coffee?. Wu, X,Skog, K,Jagerstad, M.

作者其他论文 更多>>

微信小程序