Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping

文献类型： 外文期刊

第一作者： Zhao, Jieyu

作者： Zhao, Jieyu;Li, Lili;Zhao, Yanni;Zhao, Chunxia;Zhang, Junjie;Hu, Chunxiu;Lu, Xin;Xu, Guowang;Zhao, Jieyu;Peng, Xiaojun;Chen, Xia;Liu, Pingping;Zhou, Huina;Chen, Aiguo;Liu, Guanshan

作者机构：

关键词： Topping;Tobacco leaf;Metabolomics;Liquid chromatography-mass spectrometry;Gas chromatography-mass spectrometry;Capillary electrophoresis-mass spectrometry

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2018 年 410 卷 3 期

页码：

收录情况： SCI

摘要： As an important cultivation practice used for flue-cured tobacco, topping affects diverse biological processes in the later stages of development and growth. Some studies have focused on using tobacco genes to reflect the physiological changes caused by topping. However, the complex metabolic shifts in the leaf resulting from topping have not yet been investigated in detail. In this study, a comprehensive metabolic profile of primary, secondary, and lipid metabolism in flue-cured tobacco leaf was generated with use of a multiple platform consisting of gas chromatography-mass spectrometry, capillary electrophoresis-mass spectrometry, and liquid chromatography-mass spectrometry/ultraviolet spectroscopy. A total of 367 metabolites were identified and determined. Both principal component analysis and the number of significantly different metabolites indicated that topping had the greatest influence on the upper leaves. During the early stage of topping, great lipid level variations in the upper leaves were observed, and antioxidant defense metabolites were accumulated. This indicated that the topping activated lipid turnover and the antioxidant defense system. At the mature stage, lower levels of senescence-related metabolites and higher levels of secondary metabolites were found in the topped mature leaves. This implied that topping delayed leaf senescence and promoted secondary metabolite accumulation. This study provides a global view of the metabolic perturbation in response to topping.

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