Biotechnical paving of recombinant enterocin A as the candidate of anti-Listeria agent

文献类型： 外文期刊

第一作者： Hu, Xiaoyuan

作者： Hu, Xiaoyuan;Mao, Ruoyu;Zhang, Yong;Teng, Da;Wang, Xiumin;Xi, Di;Wang, Jianhua;Hu, Xiaoyuan;Mao, Ruoyu;Zhang, Yong;Teng, Da;Wang, Xiumin;Xi, Di;Wang, Jianhua;Hu, Xiaoyuan;Huang, Jianzhong

作者机构：

关键词： Enterocin A;Listeria ivanovii;Antimicrobial activity;Pichia pastoris;Expression

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2014 年 14 卷

页码：

收录情况： SCI

摘要： Background: Enterocin A is a classic IIa bacteriocin isolated firstly from Enterococcus faecium CTC492 with selective antimicrobial activity against Listeria strains. However, the application of enterocin A as an anti-Listeria agent has been limited due to its very low native yield. The present work describes high production of enterocin A through codon optimization strategy and its character study. Results: The gene sequence of enterocin A was optimized based on preferential codon usage in Pichia pastoris to increase its expression efficiency. The highest anti-Listeria activity reached 51,200 AU/ml from 180 mg/l of total protein after 24 h of induction in a 5-L fermenter. Recombinant enterocin A (rEntA), purified by gel filtration chromatography, showed very strong activity against Listeria ivanovii ATCC 19119 with a low MIC of 20 ng/ml. In addition, the rEntA killed over 99% of tested L. ivanovii ATCC19119 within 4 h when exposed to 4 x MIC (80 ng/ml). Moreover, it showed high stability under a wide pH range (2-10) and maintained full activity after 1 h of treatment at 80 degrees C within a pH range of 2-8. Its antimicrobial activity was enhanced at 25 and 50 mM NaCl, while 100-400 mM NaCl had little effect on the bactericidal ability of rEntA. Conclusion: The EntA was successfully expressed in P. pastoris, and this feasible system could pave the pre-industrial technological path of rEntA as a competent candidate as an anti-Listeria agent. Furthermore, it showed high stability under wide ranges of conditions, which could be potential as the new candidate of anti-Listeria agent.

分类号：

  • 相关文献

[1]Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris. Wang, Tingting,Xu, Yongping,Liu, Wenjia,Jin, Liji,Xu, Yongping,Jin, Liji,Sun, Yongxin,Wang, Tingting,Xu, Yongping,Liu, Wenjia,Sun, Yongxin,Jin, Liji.

[2]Recombinant production of the antimicrobial peptide NZ17074 in Pichia pastoris using SUMO3 as a fusion partner. Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H.,Wang, X. J.,Wang, X. M.,Teng, D.,Zhang, Y.,Mao, R. Y.,Wang, J. H..

[3]Molecular analysis and recombinant expression of bovine neutrophil beta-defensin 12 and its antimicrobial activity. Wu, Jianming,Wang, Changfa,He, Hongbin,Yang, Hongjun,Gao, Yundong,Zhong, Jifeng,Hu, Guixue. 2011

[4]Discovery and characterization of Coturnix chinensis avian ss-defensin 10, with broad antibacterial activity. Ma, Deying,Lin, Lijuan,Zhang, Kexin,Wang, Ruiqin,Lin, Lijuan,Zhang, Kexin,Han, Zongxi,Shao, Yuhao,Wang, Ruiqin,Liu, Shengwang. 2012

[5]Identification and characterization of an avian beta-defensin orthologue, avian beta-defensin 9, from quails. Wang, Ruiqin,Ma, Deying,Lin, Lijuan,Zhou, Caiyuan,Liao, Wenyan,Han, Zongxi,Shao, Yuhao,Liu, Shengwang,Liu, Shengwang. 2010

[6]Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris. Wang, Lixin,Bai, Junjie,Luo, Jianren,Chen, Hong,Ye, Xing,Jian, Qing,Lao, Haihua. 2007

[7]Expression of tachyplesin gene in yeast Pichia pastoris. Zhang, CY,Zhao, J,Wu, CJ,Fan, YL. 1998

[8]Cloning and overexpression of a Paenibacillus beta-glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. Yang, Peilong,Shi, Pengjun,Wang, Yaru,Bai, Yingguo,Meng, Kun,Luo, Huiying,Yuan, Tiezheng,Yao, Bin. 2007

[9]Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Yuan, Tiezheng,Yang, Peilong,Wang, Yaru,Meng, Kun,Luo, Huiying,Yao, Bin,Zhang, Wei,Wu, Ningfeng,Fan, Yunliu.

[10]High-level Expression of a Manganese Superoxide Dismutase (PoMn-SOD) from Pleurotus ostreatus in Pichia pastoris. Yin, Chaomin,Zhao, Wenxia,Ma, Aimin,Zheng, Liesheng,Chen, Liguo,Tan, Qi,Shang, Xiaodong.

[11]A novel highly acidic beta-mannanase from the acidophilic fungus Bispora sp MEY-1: gene cloning and overexpression in Pichia pastoris. Luo, Huiying,Wang, Yaru,Wang, Hui,Yang, Jun,Yang, Yuhui,Huang, Huoqing,Yang, Peilong,Bai, Yingguo,Shi, Pengjun,Yao, Bin,Fan, Yunliu.

[12]Deleting the first disulphide bond in an arenicin derivative enhances its expression in Pichia pastoris. Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Feng, X..

[13]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[14]High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Yanqun,Wang, Gang,Wu, Jianan,Liu, Yonggang,Wang, Shujie,Jiang, Chenggang,Cai, Xuehui.

[15]Cloning, Expression, and Characterization of a Milk-Clotting Aspartic Protease Gene (Po-Asp) from Pleurotus ostreatus. Yin, Chaomin,Ma, Aimin,Zheng, Liesheng,Chen, Liguo,Tan, Qi,Shang, Xiaodong.

[16]Design, expression and characterization of the hybrid antimicrobial peptide LHP7, connected by a flexible linker, against Staphylococcus and Streptococcus. Xi, Di,Xiang, Wensheng,Xi, Di,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Yalin,Wang, Jianhua,Xi, Di,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Yalin,Wang, Jianhua. 2013

[17]Isolation of a Novel Lipase Gene from Serratia liquefaciens S33 DB-1, Functional Expression in Pichia pastoris and its Properties. Yao, Hongyan,Yu, Shunwu,Zuo, Kaijing,Ling, Hua,Zhang, Fei,Tang, Kexuan,Yu, Shunwu.

[18]Expression, purification and characterization of WSSV-VP37 in Pichia pastoris. Liu Qing-hui,Huang Jie,Han Wen-jun,Liang Yan,Lu Chun-ling,Wang Qing-yin. 2006

[19]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[20]Expression of Prolactin Receptor mRNA after Melatonin Manipulated in Cashmere Goats Skin during Cashmere Growth. Zhang, Wei,Zhu, Xiaoping,Jia, Zhihai,Yue, Chunwang,Kong, Xianghao,Du, Lixin.

作者其他论文 更多>>

微信小程序