The genome of Paenibacillus sabinae T27 provides insight into evolution, organization and functional elucidation of nif and nif-like genes

文献类型： 外文期刊

第一作者： Li, Xinxin

作者： Li, Xinxin;Deng, Zhiping;Liu, Zhanzhi;Wang, Tianshu;Xie, Jianbo;Chen, Sanfeng;Li, Xinxin;Chen, Sanfeng;Yan, Yongliang;Lin, Min;Cheng, Qi

作者机构：

关键词： Paenibacillus sabinae T27;nif gene cluster;nif-like gene;Genome sequence

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: Most biological nitrogen fixation is catalyzed by the molybdenum nitrogenase. This enzyme is a complex which contains the MoFe protein encoded by nifDK and the Fe protein encoded by nifH. In addition to nifHDK, nifHDK-like genes were found in some Archaea and Firmicutes, but their function is unclear. Results: We sequenced the genome of Paenibacillus sabinae T27. A total of 4,793 open reading frames were predicted from its 5.27 Mb genome. The genome of P. sabinae T27 contains fifteen nitrogen fixation (nif) genes, including three nifH, one nifD, one nifK, four nifB, two nifE, two nifN, one nifX and one nifV. Of the 15 nif genes, eight nif genes (nifB, nifH, nifD, nifK, nifE, nifN, nifX and nifV) and two non-nif genes (orf1 and hesA) form a complete nif gene cluster. In addition to the nif genes, there are nitrogenase-like genes, including two nifH-like genes and five pairs of nifDK-like genes. IS elements on the flanking regions of nif and nif-like genes imply that these genes might have been obtained by horizontal gene transfer. Phylogenies of the concatenated 8 nif gene (nifB, nifH, nifD, nifK, nifE, nifN, nifX and nifV) products suggest that P. sabinae T27 is closely related to Frankia. RT-PCR analysis showed that the complete nif gene cluster is organized as an operon. We demonstrated that the complete nif gene cluster under the control of sigma(70)-dependent promoter enabled Escherichia coli JM109 to fix nitrogen. Also, here for the first time we demonstrated that unlike nif genes, the transcriptions of nifHDK-like genes were not regulated by ammonium and oxygen, and nifH-like or nifD-like gene could not restore the nitrogenase activity of Klebsiella pneumonia nifH-and nifD-mutant strains, respectively, suggesting that nifHDK-like genes were not involved in nitrogen fixation. Conclusions: Our data and analysis reveal the contents and distribution of nif and nif-like genes and contribute to the study of evolutionary history of nitrogen fixation in Paenibacillus. For the first time we demonstrated that the transcriptions of nifHDK-like genes were not regulated by ammonium and oxygen and nifHDK-like genes were not involved in nitrogen fixation.

分类号：

  • 相关文献

[1]Genome-Wide Discovery of Microsatellite Markers from Diploid Progenitor Species, Arachis duranensis and A. ipaensis, and Their Application in Cultivated Peanut (A. hypogaea). Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Qiu, Jingjing,Ma, Changle,Wang, Xingjun,Agarwal, Gaurav,Guo, Baozhu,Agarwal, Gaurav,Varshney, Rajeev K.,Pandey, Manish K.,Bertioli, David J.. 2017

[2]Increased novel single nucleotide polymorphisms in weedy rice populations associated with the change of farming styles: Implications in adaptive mutation and evolution. Song, Dong-Yuan,Wang, Zhe,Song, Zhuo-Jun,Zhou, Cheng-Chuan,Xu, Peng-Hao,Yang, Ji,Lu, Bao-Rong,Yang, Jie. 2017

[3]Complete genome sequence of Ostreid herpesvirus-1 associated with mortalities of Scapharca broughtonii broodstocks. Xia, Junyang,Xia, Junyang,Bai, Changming,Wang, Chongming,Song, Xiaoling,Huang, Jie,Bai, Changming,Wang, Chongming,Song, Xiaoling,Huang, Jie. 2015

[4]The complete chloroplast genome of Lilium taliense, an endangered species endemic to China. Zhang, Qian,Zhang, Mingfang,Chen, Xuqing,Yang, Fengping,Xue, Jing,Du, Yunpeng,Zhang, Xiuhai,Bi, Yu.

[5]Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster. Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan,Weng, Yiqun,Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan.

[6]Complete genome sequence of Bacillus amyloliquefaciens L-S60, a plant growth-promoting and antifungal bacterium. Qin, Yuxuan,Han, Yuzhu,Zhang, Bao,Li, Pinglan,Yu, Yaqiong,Shang, Qingmao.

[7]A Novel Recombined Potato virus Y Isolate in China. Han, Shuxin,Gao, Yanling,Zhang, Wei,Zhang, Junhua,Gao, Yanling,Fan, Guoquan,Zhang, Wei,Qiu, Cailing,Zhang, Shu,Bai, Yanju,Spetz, Carl. 2017

[8]IDENTIFICATION OF GENOME RECOMBINATION AMONG APPLE STEM PITTING VIRUS ISOLATES. Li, Z.,Liu, P.,Sun, L.,Wu, Y.,Li, Z.,Liu, P.,Sun, L.,Wu, Y.,Li, Z.,Kondo, H.,Andika, I. B..

[9]Draft genome sequence of Pseudomonas oleovorans strain MGY01 isolated from deep sea water. Wang, Runping,Zeng, Runying,Wang, Runping,Ren, Chong,Huang, Nan,Liu, Yang,Zeng, Runying.

[10]Draft genome sequence of algal polysaccharides degradation bacterium, Flammeovirga sp OC4. Liu, Yang,Yi, Zhiwei,Zeng, Runying,Zeng, Runying,Cai, Yaping.

[11]The complete genome sequence of Citrus vein enation virus from China. Huang Ai-jun,Song Zhen,Cao Meng-ji,Chen Hong-ming,Li Zhong-an,Zhou Chang-yong,Huang Ai-jun. 2015

[12]Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean. Wu, Jing,Wang, Lanfen,Wang, Shumin,Chen, Jibao. 2016

[13]Bolbase: a comprehensive genomics database for Brassica oleracea. Yu, Jingyin,Zhao, Meixia,Tong, Chaobo,Huang, Shunmou,Tehrim, Sadia,Hua, Wei,Liu, Shengyi,Wang, Xiaowu,Liu, Yumei,Zhao, Meixia. 2013

[14]Complete genome sequence of Pseudomonas azotoformans S4, a potential biocontrol bacterium. Fang, Yang,Wu, Lijuan,Chen, Guoqing,Feng, Guozhong,Fang, Yang.

[15]Complete genome sequence of Streptomyces griseochromogenes ATCC 14511(T), a producer of nucleoside compounds and diverse secondary metabolites. Wu Lijuan,Chen, Guoqing,Feng, Guozhong.

[16]Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation. Chen, Mingli,Wu, Jing,Wang, Lanfeng,Jia, Jizeng,Wang, Shumin,Zhang, Xiaoyan,Blair, Matthew W..

[17]Complete genome sequence of an aromatic compound degrader Arthrobacter sp YC-RL1. Ren, Lei,Jia, Yang,Ruth, Nahurira,Zhao, Baisuo,Yan, Yanchun.

[18]Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China. Cheng, Yuening,Wang, Jianke,Zhang, Miao,Zhao, Jianjun,Shao, Xiqun,Zhao, Hang,Lin, Peng,Wu, Hua,Ma, Zengjun.

[19]Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China. Li Shi-fang,Lu Mei-guang. 2016

[20]Genome-wide identification and analysis of the MADS-box gene family in sesame. Wei, Xin,Wang, Linhai,Yu, Jingyin,Zhang, Yanxin,Li, Donghua,Zhang, Xiurong.

作者其他论文 更多>>

微信小程序