Self-Assembly and Release of Peste des Petits Ruminants Virus-Like Particles in an Insect Cell-Baculovirus System and Their Immunogenicity in Mice and Goats

文献类型： 外文期刊

第一作者： Li, Wenchao

作者： Li, Wenchao;Jin, Hongyan;Sui, Xiukun;Zhao, Zhanzhong;Li, Gang;Li, Wenchao;Jin, Hongyan;Sui, Xiukun;Zhao, Zhanzhong;Li, Gang;Li, Wenchao;Jin, Hongyan;Sui, Xiukun;Zhao, Zhanzhong;Li, Gang;Yang, Chenghuai;Li, Junping;Wang, Wenquan

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 8 期

页码：

收录情况： SCI

摘要： Peste des petits ruminants (PPR) is an acute, febrile, viral disease of small ruminants that has a significant economic impact. For many viral diseases, vaccination with virus-like particles (VLPs) has shown considerable promise as a prophylactic approach; however, the processes of assembly and release of peste des petits ruminants virus (PPRV) VLPs are not well characterized, and their immunogenicity in the host is unknown. In this study, VLPs of PPRV were generated in a baculovirus system through simultaneous expression of PPRV matrix (M) protein and hemaglutin in (H) or fusion (F) protein. The released VLPs showed morphology similar to that of the native virus particles. Subcutaneous injection of these VLPs (PPRV-H, PPRV-F) into mice and goats elicited PPRV specific IgG production, increased the levels of virus neutralizing antibodies, and promoted lymphocyte proliferation. Without adjuvants, the immune response induced by the PPRV H VLPs was comparable to that obtained using equivalent amounts of PPRV vaccine. Thus, our results demonstrated that VLPs containing PPRV-M protein and H or F protein are potential "differentiating infected from vaccinated animals'' (DIVA) vaccine candidates for the surveillance and eradication of PPR.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序