Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate

文献类型： 外文期刊

第一作者： Hua, Rong-Hong

作者： Hua, Rong-Hong;Li, Ye-Nan;Chen, Zhen-Shi;Liu, Li-Ke;Huo, Hong;Wang, Xiao-Lei;Guo, Li-Ping;Shen, Nan;Wang, Jing-Fei;Bu, Zhi-Gao

作者机构：

关键词： Japanese encephalitis virus;Mammalian cell line;Virus-like particle;Subunit vaccine

期刊名称：BMC BIOTECHNOLOGY （ 影响因子：2.563； 五年影响因子：3.292 ）

ISSN： 1472-6750

年卷期： 2014 年 14 卷

页码：

收录情况： SCI

摘要： Background: Japanese encephalitis virus (JEV) is the most important cause of epidemic encephalitis in most Asian regions. There is no specific treatment available for Japanese encephalitis, and vaccination is the only effective way to prevent JEV infection in humans and domestic animals. The purpose of this study is to establish a new mammalian cell line stably and efficiently expressing virus-like particle of JEV for potential use of JEV subunit vaccine. Results: We generated a new cell clone (BJ-ME cells) that stably produces a secreted form of Japanese encephalitis virus (JEV) virus-like particle (VLP). The BJ-ME cells were engineered by transfecting BHK-21 cells with a code-optimized cDNA encoding JEV prM and E protein expression plasmid. Cell line BJ-ME can stably produces a secreted form of Japanese encephalitis virus virus-like particle (JEV-VLP) which contains the JEV envelope glycoprotein (E) and membrane protein (M). The amount of JEV-VLP antigen released into the culture fluid of BJ-ME cells was as high as 15-20 mu g/ml. JEV-VLP production was stable after multiple cell passages and 100% cell expression was maintained without detectable cell fusion or apoptosis. Cell culture fluid containing the JEV-VLP antigen could be harvested five to seven times continuously at intervals of 4-6 days while maintaining the culture. Mice immunized with the JEV-VLP antigen with or without adjuvant developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge. Conclusion: These results suggest that the recombinant JEV-VLP antigen produced by the BJ-ME cell line is an effective, safe and affordable subunit Japanese encephalitis vaccine candidate, especially for domestic animals such as pig and horse.

分类号：

  • 相关文献

[1]Function of VP2 Protein in the Stability of the Secondary Structure of Virus-like Particles of Genogroup II Norovirus at Different pH Levels: Function of VP2 Protein in the Stability of NoV VLPs. Yao Lin,Li Fengling,Wang Lianzhu,Zhai Yuxiu,Jiang Yanhua. 2014

[2]Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits. Guo, Huimin,Zhu, Jie,Tan, Yonggui,Li, Chuanfeng,Chen, Zongyan,Liu, Guangqing,Sun, Shiqi.

[3]Newcastle disease virus-like particles induce dendritic cell maturation and enhance viral-specific immune response. Qian, Jing,Ding, Jiaxin,Yin, Renfu,Xue, Cong,Xu, Xiaohong,Cong, Yanlong,Ding, Zhuang,Qian, Jing,Ding, Jiaxin,Yin, Renfu,Xue, Cong,Xu, Xiaohong,Cong, Yanlong,Ding, Zhuang,Sun, Yixue,Wang, Jianzhong,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Hu, Shunlin.

[4]Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice. Gai, Weiwei,Zhang, Weijiao,Qiu, Boning,Gai, Weiwei,Wang, Hualei,Zhao, Yongkun,Wang, Qi,Zhang, Weijiao,Feng, Na,Qiu, Boning,Chi, Hang,Li, Nan,Wang, Tiecheng,Gao, Yuwei,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Wang, Chong,Wang, Qi,Wong, Gary,Shan, Junjie,Shan, Junjie,Yang, Songtao,Xia, Xianzhu. 2017

[5]Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV. Wang, Yong-shan,Ouyang, Wei,Liu, Xiao-juan,He, Kong-wang,Liu, Xiao-juan,Zhang, Hai-bin,Fan, Hong-jie,Lu, Cheng-ping,Yu, Sheng-qing. 2012

[6]Immunization by influenza virus-like particles protects aged mice against lethal influenza virus challenge. Wen, Zhiyuan,Ye, Ling,Gao, Yulong,Pan, Lei,Dong, Ke,Compans, Richard W.,Yang, Chinglai,Wen, Zhiyuan,Ye, Ling,Gao, Yulong,Pan, Lei,Dong, Ke,Compans, Richard W.,Yang, Chinglai,Wen, Zhiyuan,Gao, Yulong,Bu, Zhigao,Pan, Lei,Dong, Ke. 2009

[7]Immunoprotection induced by CpG-ODN/Poly(I:C) combined with recombinant gp90 protein in chickens against reticuloendotheliosis virus infection. Yuan, Fei,Chu, Ying,Li, Hongmei,Sun, Shuhong,Zhao, Peng,Chang, Shuang,Guo, Huijun,Qi, Lihong. 2017

[8]Identification of novel Haemophilus parasuis serovar 5 vaccine candidates using an immunoproteomic approach. Li, Gang,Xie, Fang,Li, Jianjun,Liu, Jiao,Li, Dapeng,Zhang, Yanhe,Liu, Siguo,Wang, Chunlai,Langford, Paul R.,Li, Yanwen.

[9]Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins. Liu, Yan,Chen, Hui,Wei, Qiang,Xiao, Chenwen,Ji, Quanan,Bao, Guolian. 2015

[10]Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[11]Recombinant gp90 protein expressed in Pichia pastoris induces a protective immune response against reticuloendotheliosis virus in chickens. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei. 2012

[12]Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae. Li ZhiYong,Yi YongZhu,Yin XiangPing,Zhang ZhiFang,Liu JiXing.

[13]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[14]mosGCTL-7, a C-Type Lectin Protein, Mediates Japanese Encephalitis Virus Infection in Mosquitoes. Liu, Ke,Qian, Yingjuan,Jung, Yong-Sam,Zhou, Bin,Cao, Ruibing,Shen, Ting,Chen, Puyan,Zhu, Huaimin,Liu, Ke,Shao, Donghua,Wei, Jianchao,Ma, Zhiyong,Qiu, Yafeng.

[15]Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection. Ishag, Hassan Z. A.,Li, Chen,Wang, Fengjuan,Mao, Xiang,Mao, Xiang,Ishag, Hassan Z. A..

[16]Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China. Fan, Jian-Ming,Luo, Jun,Teng, Man,Bu, Dan,Wang, Fang-Yu,Wang, Li,Zhang, Gai-Ping,Fan, Jian-Ming,Chen, Lu,Wang, Chuan-Qing. 2010

[17]Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model. Shi, Zixue,Wei, Jianchao,Deng, Xufang,Qiu, Yafeng,Shao, Donghua,Li, Beibei,Zhang, Keyu,Xue, Feiqun,Ma, Zhiyong,Li, Shuqing,Wang, Xiaodu. 2014

[18]Porcine alpha interferon inhibit Japanese encephalitis virus replication by different ISGs in vitro. Liao, Xuewen,Yao, Huijuan,Fan, Shengchao,Miao, Denian,Liu, Ke,Zhou, Bin,Chen, Puyan,Liu, Ke. 2013

[19]A simple method for developing an infectious cDNA clone of Japanese encephalitis virus. Zheng, Hao,Zheng, Xuchen,Tong, Wu,Liu, Fei,Liang, Chao,Wang, Tao,Gao, Fei,Li, Liwei,Shan, Tongling,Li, Guoxin,Tong, Guangzhi,Zheng, Hao,Tong, Wu,Tong, Guangzhi.

[20]Antibodies generated by immunization with the NS1 protein of West Nile virus confer partial protection against lethal Japanese encephalitis virus challenge. Sun, EnCheng,Zhao, Jing,TaoYang,Xu, QingYuan,Qin, YongLi,Wang, WenShi,Wei, Peng,Wu, DongLai. 2013

作者其他论文 更多>>

微信小程序