Endophytic nitrogen-fixing Klebsiella variicola strain DX120E promotes sugarcane growth

文献类型： 外文期刊

第一作者： Wei, Chun-Yan

作者： Wei, Chun-Yan;Lin, Li;Luo, Li-Jing;Xing, Yong-Xiu;Yang, Li-Tao;Li, Yang-Rui;Lin, Li;Hu, Chun-Jin;Li, Yang-Rui;Lin, Li;Hu, Chun-Jin;Li, Yang-Rui;Lin, Li;Hu, Chun-Jin;Li, Yang-Rui;Lin, Li;Hu, Chun-Jin;Li, Yang-Rui;Lin, Li;Hu, Chun-Jin;Li, Yang-Rui;An, Qianli

作者机构：

关键词： Colonization;Endophytic bacteria;Klebsiella variicola;Nitrogen fixation;Plant growth-promoting bacteria;Sugarcane

期刊名称：BIOLOGY AND FERTILITY OF SOILS （ 影响因子：6.432； 五年影响因子：6.332 ）

ISSN： 0178-2762

年卷期： 2014 年 50 卷 4 期

页码：

收录情况： SCI

摘要： Klebsiella variicola bacteria are found in association with plants. Little is known about their colonization patterns, roles, and mechanisms during association with the plant hosts. Here, we identified a nitrogen-fixing bacterium, DX120E, which was isolated from surface-sterilized roots of the ROC22 sugarcane cultivar, as K. variicola by phylogenetic analyses of its 16S rRNA gene, RNA polymerase beta-subunit gene, and DNA gyrase subunit A gene sequences. gfp-tagged DX120E was found to colonize at the roots and aerial parts of micropropagated sugarcane plantlets by fluorescence microscopy and confocal microscopy. DX120E was able to survive in soils and colonize in root epidermal cells, intercellular spaces in root cortices, and leaf mesophyll and vascular tissues. DX120E preferentially colonized at root maturation and elongation zones and entered roots via cracks at the emergence site of lateral roots and at disrupted epidermis. DX120E may penetrate root epidermal cells with the aid of their cellulose-degrading enzymes. N-15 isotope dilution assay demonstrated that DX120E was able to fix N-2 in association with ROC22 sugarcane plants under gnotobiotic condition. DX120E was also able to promote GT21 cultivar growth and plant uptake of N, P, and K under greenhouse condition. Together, this study for the first time shows that a K. variicola strain is able to colonize in its sugarcane plant hosts, to fix N-2 in association with plants, and to promote plant growth.

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