Adjuvant effect enhancement of porcine interleukin-2 packaged into solid lipid nanoparticles

文献类型： 外文期刊

第一作者： Chen, Guohua

作者： Chen, Guohua;Zeng, Shuang;Jia, Huaijie;He, Xiaobing;Fang, Yongxiang;Jing, Zhizhong;Cai, Xuepeng

作者机构：

关键词： Recombinant porcine interleukine-2;Solid liquid nanoparticles;Packaging;Adjuvant effect

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2014 年 96 卷 1 期

页码：

收录情况： SCI

摘要： In this paper, we investigated the enhancement of adjuvant effects of porcine IL-2 (pIL-2) by packaging it into a solid lipid nanoparticle (SLN) delivery system. SLN-pIL-2 was prepared using hydrogenated castor oil and Polylactide-co-glycolide by double emulsion solvent evaporation methods (w/o/w). In animal trials, BALB/c mice were immunized with inactivated foot and mouth disease virus (FMDV) antigen combined with the SLN-pIL-2 adjuvant on days 0 and 14. Antibody titer, splenocyte proliferation, and secretion of IFN-gamma and IL-4 cytokines were determined. Our results showed that SLN-pIL-2 could significantly enhance FMDV-specific antibody level compared with recombinant pIL-2 alone (p < 0.05). In addition, SLN-pIL-2 significantly increased the proliferative responses of antigen-specific spleen cells. Furthermore, SLN-pIL-2 induced the secretion of IFN-gamma at a level higher than that induced by recombinant pIL-2 alone. Our results indicate that packaging recombinant pIL-2 in SLNs can be an effective way of boosting the effectiveness of pIL-2 as an adjuvant to enhance immune responses of vaccines. (C) 2013 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Adjuvant Effects of Recombinant Plasmids of Porcine IL-4 and IFN-gamma to Cysticercosis cellulosae Vaccines in Mice and Pigs. Jing Zhi-zhong,Dou Yong-xi,Meng Xue-lian,Chen Guo-hua,Wang Pei-ya,Luo Qi-hui,Yuan Gai-ling,Zheng Ya-dong,Cai Xue-peng. 2010

[2]Accumulation of Lignin and Malondialdehyde in Relation to Quality Changes of Button Mushrooms (Agaricus bisporus) Stored in Modified Atmosphere. Jiang, Tianjia,Jahangir, Muhammad Muzammil,Ying, Tiejin,Wang, Qiushuang. 2010

[3]Effect of packaging on quality and flavor of fresh-cut mint. Zhang, Chao. 2015

[4]Plasmids driven minigenome rescue system for Newcastle disease virus V4 strain. Jiang, Yanlong,Liu, Huairan,Liu, Peixin,Kong, Xiangang,Jiang, Yanlong.

[5]Electrospun poly(vinyl alcohol)/permutite fibrous film loaded with cinnamaldehyde for active food packaging. Shao, Ping,Yan, Zhipeng,Chen, Hangjun,Xiao, Jie. 2018

[6]Development of an automatic milk standards packaging system. Li, Y,Ruan, R,Li, J,Yu, Q,Lin, X,Chen, P,Deng, S,Grounli, B. 2005

[7]Relation between Types of Packaging and Lipid Oxidation in Walnut (Carya cathayensis Sarg.). Tao, Fei,Gao, Haiyan,Chen, Hangjun,Chen, Wenxuan,Song, Lili,Ge, Linmei. 2008

[8]Effect of packaging on quality of fresh-cut thyme. Zhang, C.. 2015

[9]Effect of Packaging on Quality and Flavor of Fresh-Cut Perilla. Zhang, Chao,Li, Yunfei,Wang, Yubin,Ma, Yue,Zhao, Xiaoyan. 2015

作者其他论文 更多>>

微信小程序