Effects of Environmental Temperature on the Regeneration Frequency of the Immature Embryos of Wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Wang Xin-min

作者： Wang Xin-min;Yin Gui-xiang;Wang Ke;Du Li-pu;Xu Hui-jun;Ye Xing-guo;Ren Xian;Li Jia-rui

作者机构：

关键词： wheat;immature embryos;environmental temperature;preservation;plant regeneration

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4 degrees C daily cooler for a short period time for its use in plant tissue culture or transformation experiments. This study aimed to investigate the effects of environmental temperature, cryopreservation storage temperature, and heat shock culture (HSC) temperature on the regeneration frequency of wheat IEs. Results indicated that environmental temperature significantly affected the induction of embryonic calli. The optimum total accumulated temperature (TAT) during the time of anthesis and sampling for regeneration of these tissues was around 280 degrees C for spring wheat type cv. CB037 and approximately 300 degrees C for winter wheat type cv. Kenong 199. Regeneration ability obviously declined when the highest environmental temperature was over 35 degrees C for 1 d or a high temperature between 30 and 33 degrees C lasted for 5 d during anthesis and sampling. This finding was verified by culturing the freshly isolated IEs under different temperatures from 29 to 37 degrees C in different controlled growth incubators for 5 d; the IEs almost completely lost regeneration ability when the temperature rose to 37 degrees C. Cryopreservation of -20 degrees C caused the wheat samples lost ability of producing callus or embryonic callus in a few days, and cryopreservation of -10 degrees C more than 10 d made the regeneration potential of the tissues dramatically declined. Comparatively, the temperature that best maintained high regeneration ability was -5 degrees C, at which the materials can be maintained for around 1 mon. In addition, the preservation of the immature samples at -5 or -10 degrees C inhibited the direct germination of the IEs, avoiding the embryo axis removing process. Our results are useful for ensuring that field collection and cryopreservation of the wheat IEs are done correctly to enable tissue culture and generic transformation.

分类号：

  • 相关文献

[1]Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos. Zhang Wei,Wang Xin-min,Yin Gui-xiang,Wang Ke,Du Li-pu,Xiao Le-le,Ye Xing-guo,Fan Rong. 2015

[2]Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens. Tao Li-li,Yin Gui-xiang,Du Li-pu,Shi Zheng-yuan,She Mao-yun,Xu Hui-jun,Ye Xing-guo. 2011

[3]EFFECTS OF SOIL DROUGHT STRESS ON PLANT REGENERATION EFFICIENCY AND ENDOGENOUS HORMONE LEVELS OF IMMATURE EMBRYOS IN WHEAT (&ITTRITICUM AESTIVUM&IT L.). Bie, Xiaomin,Wang, Ke,Liu, Chang,Du, Lipu,Mao, Xinguo,Ye, Xingguo,Bie, Xiaomin,Liu, Yongwei. 2017

[4]Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Chen, Peidu.

[5]Seasonal variation in the biocontrol efficiency of bacterial wilt is driven by temperature-mediated changes in bacterial competitive interactions. Huang, Jianfeng,Yang, Tianjie,Jousset, Alexandre,Xu, Yangchun,Shen, Qirong,Huang, Jianfeng,Yang, Tianjie,Jousset, Alexandre,Friman, Ville-Petri.

[6]Response of rectal temperature of broiler chickens to thermal environment factors. Lin, H,Buyse, J,Du, R,Gu, XH,Zhang, ZY. 2004

[7]Somatic embryogenesis from thin epidermal layers in sunflower and chromosomal regions controlling the response. Huang, XianQun,Nabipour, Alireza,Gentzbittel, Laurent,Sarrafi, Ahmad.

[8]Comparison of dynamic changes of endogenous hormones between calli derived from mature and immature embryos of maize. Wang, Henning,Zhang, Jinwen,Jia, Xiaoxia,Liang, Huiguang,Kong, Weiping,Zhang, Xinhui. 2011

[9]The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Xing, L.,Chen, L. J.,Han, L. J.,Xing, L..

[10]Inhibitory effects of chitosan combined with nisin on Shewanella spp. isolated from Pseudosciaena crocea. He, Mu,Guo, Quan-you,Song, Wei,Zhang, Guo-wen,Li, Bao-guo.

[11]Chestnut Genebank in China National Clonal Plant Germplasm Repository. Li Guo-tian,Ai Cheng-xiang,Zhang Li-si,Wei Hai-rong,Liu Qing-zhong. 2009

[12]Effect of Poly-epsilon-lysine on vacuum packed tilapia stored at 4 degrees C. Ma, Haixia,Cheng, Linli,Li, Laihao,Yang, Xianqing. 2014

[13]Tea polyphenols incorporated into alginate-based edible coating for quality maintenance of Chinese winter jujube under ambient temperature. Zhang, Lihua,Dong, Yu,Zhi, Huanhuan,Zong, Wei,Zhang, Lihua,Dong, Yu,Zhi, Huanhuan,Zong, Wei,Li, Shunfeng. 2016

[14]Diversity of the Predominant Spoilage Bacteria in Water-Boiled Salted Duck during Storage. Liu, Fang,Wang, Daoying,Zhu, Yongzhi,Xu, Weimin,Du, Lihui. 2010

[15]Plant regeneration via somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Chang-Bing Yao. 2000

[16]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[17]Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos. Wu, Shen-Jie,Wang, Hai-Hai,Li, Fei-Fei,Chen, Tian-Zi,Zhang, Jie,Jiang, Yan-Jie,Ding, Yezhang,Guo, Wang-Zhen,Zhang, Tian-Zhen,Wu, Shen-Jie.

[18]Efficient regeneration and transformation of Spathiphyllum cannifolium. Yu, Bo,Liao, Feixiong,Liu, Jinmei,Sun, Yingbo,Huang, Lili.

[19]Hydrated mucilage reduces post-dispersal seed removal of a sand desert shrub by ants in a semiarid ecosystem. Yang, Xuejun,Gao, Ruiru,Yang, Fan,Wei, Lingling,Li, Leilei,Huang, Zhenying,Yang, Xuejun,He, Hongju,Baskin, Carol C.,Baskin, Jerry M.,Baskin, Carol C..

[20]Anther culture and plant regeneration of cotton (Gossypium klotzschianum Anderss). Feng, R,Li, XH,Li, FL. 1996

作者其他论文 更多>>

微信小程序