Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus

文献类型： 外文期刊

第一作者： Gao, Kun

作者： Gao, Kun;Deng, Xiang-yuan;Gao, Kun;Qjan, He-ying;Qin, Guangxing;Guo, Xi-jie;Gao, Kun;Qjan, He-ying;Qin, Guangxing;Guo, Xi-jie

作者机构：

关键词： Bombyx mori;Digital Gene Expression;Cytoplasmic polyhedrosis virus;Midgut

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2014 年 115 卷

页码：

收录情况： SCI

摘要： Digital Gene Expression was performed to investigate the midgut transcriptome profile of 4008 silkworm strain orally infected with BmCPV. A total of 4,498,263 and 4,258,240 clean tags were obtained from the control and BmCPV-infected larvae. A total of 752 differentially expressed genes were detected, of which 649 were upregulated and 103 were downregulated. Analysis results of the Kyoto Encyclopedia of Genes and Genomes pathway showed that 334 genes were involved in the ribosome and RNA transport pathways. Moreover, 408 of the 752 differentially expressed genes have a GO category and can be categorized into 41 functional groups according to molecular function, cellular component and biological process. Differentially expressed genes involved in signaling, gene expression, metabolic process, cell death, binding, and catalytic activity changes were detected in the expression profiles. Quantitative real-time PCR was performed to verify the expression of these genes. The upregulated expression levels of Calreticulin, FK506-binding protein, and protein kinase c inhibitor gene probably led to a calcium-dependent apoptosis in the BmCPV-infected cells. The results of this study may serve as a basis for future research not only on the molecular mechanism of BmCPV invasion but also on the anti-BmCPV mechanism of silkworm. (C) 2013 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[2]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[3]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[4]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[5]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[6]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[7]Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis. Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xiao, Sheng-Yan,Li, Nan,Shen, Zhong-Yuan.

[8]Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature. Li, Qing Rong,Xiao, Yang,Wu, Fu Quan,Ye, Ming Qiang,Luo, Guo Qing,Xing, Dong Xu,Li, Li,Yang, Qiong.

[9]Microarray analysis of the gene expression profile in the midgut of silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Wang, Xiu,Jiang, Yun-Feng,Guo, Xi-Jie,Wu, Ping,Qin, Guang-xing,Liu, Ting,Li, Mu-Wang,Guo, Xi-Jie.

[10]Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection. Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[11]Molecular cloning and characterization of biphenyl hydrolase-like (BPHL) protein gene from silkworm, Bombyx mori. Gao, Kun,Deng, Xiang Yuan,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie. 2016

[12]CDNA cloning and characterization of LASP1 from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[13]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[14]The physiological differentiation along the midgut of Bombyx mori - inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space. Qu, M.,Yang, J.,Yang, Q.,Liu, L.,Qu, M.,Yang, J.,Yang, Q.,Yang, Q.. 2018

[15]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[16]Differentially expressed genes in the ovary of the sixth day of pupal "Ming" lethal egg mutant of silkworm, Bombyx mori. Gao, Peng,Chen, An-Li,Zhao, Qiao-Ling,Shen, Xing-Jia,Qiu, Zhi-Yong,Xia, Ding-Guo,Tang, Shun-Ming,Zhang, Guo-Zheng.

[17]DGE analysis of changes in gene expression in response to temperature and deltamethrin stress in the silkworm (Bombyx mori). Tang, Xudong,Li, Nan,Wang, Wei,Yu, Jie,Xu, Li,Shen, Zhongyuan,Tang, Xudong,Xu, Li,Shen, Zhongyuan. 2016

[18]Transfection of BmCPV genomic dsRNA in silkmoth-derived Bm5 cells: Stability and interactions with the core RNAi machinery. Swevers, Luc,Kolliopoulou, Anna,Li, Zheng,Sun, Jingchen,Daskalaki, Maria,Verret, Frederic,Kalantidis, Kriton,Smagghe, Guy. 2014

[19]Down-regulation of aminopeptidase N and ABC transporter subfamily G transcripts in Cry1Ab and Cry1Ac resistant Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Wang, Yueqin,Wang, Yidong,Bai, Shuxiong,Wang, Zhenying,He, Kanglai,Coates, Brad S.. 2017

[20]Activities of Digestive Enzymes in the Omnivorous Pest Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Wu, Kongming,Zhao, Xincheng.

作者其他论文 更多>>

微信小程序