Identification of two new antigen epitopes on the putative capsid protein encoded by torque teno sus virus type 1 ORF1

文献类型： 外文期刊

第一作者： Liu, Jianbo

作者： Liu, Jianbo;Zhang, Long;Huang, Liping;Wei, Yanwu;Wu, Hongli;Liu, Changming

作者机构：

关键词： Torque teno sus virus type 1 (TTSuV1);Monoclonal antibody;Antigenepitope

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2013 年 167 卷 3-4 期

页码：

收录情况： SCI

摘要： Torque teno sus virus type 1 (ITSuV1) ORF1 is considered to encode the viral capsid (Cap) protein, which is crucial for the induction of TTSuVl-specific antibodies and protective immunity in the host. Eight monoclonal antibodies (mAbs) directed against the Cap protein were generated and biologically characterized. The immunoreactivity of the Cap protein expressed in transfected 293T cells for these mAbs was determined with an immunoperoxidase rnonolayer assay. The antigen epitopes of the Cap protein were mapped using these mAbs and truncated Cap proteins expressed in Escherichia coli. Fine epitope mapping was then performed with a panel of synthesized polypeptides. All the mAbs reacted with the Cap protein C fragment expressed in E. coli. One antigenic epitope of the Cap protein, which reacted with seven mAbs, had the polypeptide sequence (536)HPICYAGQGGGYTT(548), whereas another epitope recognized by the 1E9 mAb had the polypeptide sequence (549)EIGHQGITAASLR(561). It is interesting that the two new epitopes are adjacent, but mutually independent. This study should facilitate further investigation of the antigenic differences and enable the differential diagnosis of the virus. (C) 2013 Elsevier B.V. All rights reserved.

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