Characterization of a Maize Wip1 Promoter in Transgenic Plants

文献类型: 外文期刊

第一作者: Zhang, Shengxue

作者: Zhang, Shengxue;Liu, Yan;Wang, Xiaoqing;Liu, Yunjun;Wang, Guoying;Lian, Yun

作者机构:

关键词: Wip1;promoter;transcriptional start site;transgenic plant

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )

ISSN: 1422-0067

年卷期: 2013 年 14 卷 12 期

页码:

收录情况: SCI

摘要: The Maize Wip1 gene encodes a wound-induced Bowman-Birk inhibitor (BBI) protein which is a type of serine protease inhibitor, and its expression is induced by wounding or infection, conferring resistance against pathogens and pests. In this study, the maize Wip1 promoter was isolated and its function was analyzed. Different truncated Wip1 promoters were fused upstream of the GUS reporter gene and transformed into Arabidopsis, tobacco and rice plants. We found that (1) several truncated maize Wip1 promoters led to strong GUS activities in both transgenic Arabidopsis and tobacco leaves, whereas low GUS activity was detected in transgenic rice leaves; (2) the Wip1 promoter was not wound-induced in transgenic tobacco leaves, but was induced by wounding in transgenic rice leaves; (3) the truncated Wip1 promoter had different activity in different organs of transgenic tobacco plants; (4) the transgenic plant leaves containing different truncated Wip1 promoters had low GUS transcripts, even though high GUS protein level and GUS activities were observed; (5) there was one transcription start site of Wip1 gene in maize and two transcription start sites of GUS in Wip1::GUS transgenic lines; (6) the adjacent 35S promoter which is present in the transformation vectors enhanced the activity of the truncated Wip1 promoters in transgenic tobacco leaves, but did not influence the disability of truncated Wip(1231) promoter to respond to wounding signals. We speculate that an ACAAAA hexamer, several CAA trimers and several elements similar to ACAATTAC octamer in the 5-untranslated region might contribute to the strong GUS activity in Wip(1231) transgenic lines, meanwhile, compared to the 5-untranslated region from Wip(1231) transgenic lines, the additional upstream open reading frames (uORFs) in the 5-untranslated region from Wip(1737) transgenic lines might contribute to the lower level of GUS transcript and GUS activity.

分类号:

  • 相关文献

[1]Isolation and Functional Analysis of Convolvulus arvensis EPSPS Promoter. Wang, Guirong,Huang, Hongjuan,Wei, Shouhui,Zhou, Xinxin,Chen, Jinyi,Chen, Jingchao,Zhang, Chaoxian.

[2]Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells. Tang, Yiting,Sheng, Ming,Liu, Honglin,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Tang, Yiting,Liu, Lan,Huang, Lei,Gao, Qian,Wei, Jingliang,Wu, Tianwen,Yang, Shulin,Mu, Yulian,Li, Kui,Xiong, Kai. 2015

[3]Effects of Bt transgenic cotton lines on the cotton bollworm parasitoid Microplitis mediator in the laboratory. Liu, XX,Zhang, QW,Zhao, JZ,Li, HC,Xu, BL,Ma, XM. 2005

[4]Genetic transformation of watercress with a gene encoding for betaine-aldehyde dehydrogenase (BADH). Li, YX,Chang, FQ,Du, LQ,Guo, BH,Li, HJ,Zhang, JS,Chen, SY,Zhu, ZQ. 2000

[5]Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection. Jiang, Shanshan,Jiang, Liangliang,Jiang, Shanshan,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei. 2018

[6]Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt. Peng, YF,Cao, GC,Ma, JS,Chen, CX,Zhang, LM,Li, WB,Sun, YR. 1998

[7]Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber. Liu Li-ying,Duan Liu-sheng,Zhang Jia-chang,Zhang Xiao-lan,Zhang Zhen-xian,Ren Hua-zhong,Mi Guo-quan. 2013

[8]Efficient Sugarcane Transformation via bar Gene Selection. Wang, W. Z.,Yang, B. P.,Feng, C. L.,Wang, J. G.,Xiong, G. R.,Zhao, T. T.,Zhang, S. Z.. 2017

[9]A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis. Lu, Min,Ying, Sheng,Zhang, Deng-Feng,Shi, Yun-Su,Song, Yan-Chun,Wang, Tian-Yu,Li, Yu. 2012

[10]NAC transcription factors in plant multiple abiotic stress responses: progress and prospects. Shao, Hongbo,Shao, Hongbo,Shao, Hongbo,Wang, Hongyan,Tang, Xiaoli,Wang, Hongyan. 2015

[11]Detection of unintended effects in genetically modified herbicide-tolerant (GMHT) rice in comparison with non-target phenotypic characteristics. Xiao, Guoying,Jiang, Xianbin,Jiang, Xianbin. 2010

[12]Improved phosphorus acquisition by tobacco through transgenic expression of mitochondrial malate dehydrogenase from Penicillium oxalicum. Lu, Jun,Gao, Xiaorong,Yi, Jun,An, Lijia,Dong, Zhimin. 2012

[13]Efficacy of transgenic Bt cotton for resistance to the Asian corn borer (Lepidoptera : Crambidae). He, KL,Wang, ZY,Bai, SX,Zheng, L,Wang, YB,Cui, HY. 2006

[14]Influences of the disease resistance conferred by the individual transgenes, Pi-d2, Pi-d3 and Xa21, on the transgenic rice plants in yield and grain quality. Hao, Z. N.,Wang, L. P.,Tao, R. X.,Wang, J.,Wang, J.. 2009

[15]Developing insect resistance with fusion gene transformation of chitinase and scorpion toxin gene in maize (Zea mays L). Liu, Ming,Wang, Jingxue,Hao, Yaoshan,Sun, Yi.

[16]Recent Advances in Utilizing Transcription Factors to Improve Plant Abiotic Stress Tolerance by Transgenic Technology. Wang, Hongyan,Wang, Honglei,Shao, Hongbo,Shao, Hongbo,Tang, Xiaoli. 2016

[17]Development of monoclonal antibody-based sensitive ELISA for the determination of Cry1Ie protein in transgenic plant. Zhang, Yuwen,Wang, Guoying,Liu, Yunjun,Zhang, Yuwen,Zhang, Wei,Liu, Yan,Wang, Jianhua.

[18]Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Wang, Xiaoxue,Wu, Ningfeng,Guo, Jun,Chu, Xiaoyu,Tian, Jian,Fan, Yunliu,Yao, Bin.

[19]Over-expression of the Gr5(aroA) gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco. Wang, Jin,Lu, Wei,Zhu, Yu,Lin, Min,Zuo, Kaijing,Tang, Kexuan,Ye, Chun. 2014

[20]Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves. Wang, Limin,Wang, Xuchu,Jin, Xiang,Jia, Ruizong,Huang, Qixing,Tan, Yanhua,Guo, Anping,Wang, Limin. 2015

作者其他论文 更多>>